. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 244 ALFRED C. REDFIELD containing a convenient proportion of oxygen. In the experiments de- scribed in this paper, nitrogen containing 2 to 5 per cent of oxygen has been convenient; for other solutions, air will serve or pure oxygen. FIG. 1. Arrangement for adjusting pressure of gas in tonometers. A, tonometer in position for measuring pressure after equilibration. B, tonometer in thermostat during settling of sediment. M, mercury manometer. R, reservoir which serves to slow rate of evacuation of tonometers. may be necessary
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 244 ALFRED C. REDFIELD containing a convenient proportion of oxygen. In the experiments de- scribed in this paper, nitrogen containing 2 to 5 per cent of oxygen has been convenient; for other solutions, air will serve or pure oxygen. FIG. 1. Arrangement for adjusting pressure of gas in tonometers. A, tonometer in position for measuring pressure after equilibration. B, tonometer in thermostat during settling of sediment. M, mercury manometer. R, reservoir which serves to slow rate of evacuation of tonometers. may be necessary. The tonometer is now evacuated to some definite pressure and the solution equilibrated with the gas at this pressure, ro- tating the tonometer in a horizontal position for fifteen minutes in a water bath at constant temperature. The tonometer is next returned to a vertical position and evacuated and filled again with the gas mixture and pumped out to the same reduced pressure. The solution is further equilibrated for twenty-five minutes and then, without removal from the water bath, is turned into the vertical position and connected with the manometer after setting the pressure in the system to that expected to obtain in the tonometer. The passage connecting the tonometer and manometer with the pump and reservoir is now closed. The stopcock leading into the tonometer is opened, and the pressure obtaining in the tonometer is carefully measured and recorded. The stopcock of the tonometer is closed again and disconnected from the pump. The tonometer is carefully dried and placed in an air-chamber inserted into the water bath where it is kept for a period of approximately one hour in order that the small particles of denatured protein which almost in- variably form during the process of evacuation and equilibration may. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these il
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
