. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MARGINAL BANDS OF LOBSTER BLOOD CELLS 59. FIGURE 8. Fate of the MB during spreading as visualized by indirect immunofluorescence after anti-tubulin binding, (a, b) Five min spreading; MBs still recognizable as such. A few cells contain elliptical MBs (a, arrow), but in the majority the MBs show signs of disorganization, such as extensive twisting (b). (c) Ten min spreading; MBs are no longer recognizable. Microtubule bundles are evident, and there is a genera! background fluorescence, (d) Twenty min spreading; there are poin
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MARGINAL BANDS OF LOBSTER BLOOD CELLS 59. FIGURE 8. Fate of the MB during spreading as visualized by indirect immunofluorescence after anti-tubulin binding, (a, b) Five min spreading; MBs still recognizable as such. A few cells contain elliptical MBs (a, arrow), but in the majority the MBs show signs of disorganization, such as extensive twisting (b). (c) Ten min spreading; MBs are no longer recognizable. Microtubule bundles are evident, and there is a genera! background fluorescence, (d) Twenty min spreading; there are points of fluorescence visible, but microtubules are not present. The nucleus is also sometimes fluorescent, as in this example; it is not clear whether this is an artifact or may have some significance. Fluorescence microscopy, mag. bars as in (a). were also described as being the major cell type in the blood of the crayfish (Cam- barns bartoni) and the blue crab (Callinectes sapidus). Hearing and Vernick (1967) examined the blood cells of//, americanus by light and electron microscopy, using samples fixed immediately after removal from the animal. Neither MBs nor microtubules were observed, and apparently they were not preserved by the fixation methods employed. Three cell types were identified and counted with respect to percentage of the population: "Eosinophils" (20% of population), "ovoid basophils" (20%), and "spindular basophils" (60%). All of the cells contained granules, with the largest ones observed in the "spindular basophils". Identification of cell type was based primarily upon the examination of cells in sections, and it appears that the plane of sectioning may not have been taken into account in interpreting the images. Thus it is likely that the "spindular basophils" were flattened, oval cells cut at various angles (as in Fig. 5), and that the "ovoid basophils" were the same cells in face view (Fig. 4). T
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
