. Ciba Foundation Symposium on Transplantation. Transplantation of organs, tissues, etc. H-2 ANTIGENS OF THE MOUSE 55 cent sialic acid, but no uronic acid, although the material is markedly acidic. The products from successive batches of mice vary considerably in their proportions of lipid and protein and, taking the nitrogen content of the "lipid-free" residue as 14-5 per cent, the proportions of lipid and protein can be found from the percentage of nitrogen in the TM2 fraction. The nitrogen 1000-. Time (min.) Fig. I. The effect of ultrasonic vibration on the haemagglu- tination inh
. Ciba Foundation Symposium on Transplantation. Transplantation of organs, tissues, etc. H-2 ANTIGENS OF THE MOUSE 55 cent sialic acid, but no uronic acid, although the material is markedly acidic. The products from successive batches of mice vary considerably in their proportions of lipid and protein and, taking the nitrogen content of the "lipid-free" residue as 14-5 per cent, the proportions of lipid and protein can be found from the percentage of nitrogen in the TM2 fraction. The nitrogen 1000-. Time (min.) Fig. I. The effect of ultrasonic vibration on the haemagglu- tination inhibiting activity of H-2 antigen. content of the products of different batches has varied from 11 • o to 8- 5 per cent, with lipid from 30 to 50 per cent. The nitrogen content of the TMi fractions is slightly lower than that of the TM2. The lipid and protein components can be largely separated by solvent extraction. Removal of hpid with ether at —15°, using poorly active freeze-dried material, resulted in little loss of. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ciba Foundation Symposium on Transplantation (1961 : London, England); Wolstenholme, G. E. W. (Gordon Ethelbert Ward); Cameron, Margaret P. Boston, Little, Brown
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