. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 270 W. BELL AND R. MITCHELL medium; the gradual increase with age can be accounted for by evaporation of the medium in the flasks. It is evident that the bacteria tested are able to coexist in culture with these algae. The data suggest that most of these bacteria depend on degradation products of the algal cells. A small residual population of bacteria existed in a viable state throughout the 30-day period tested, even in control flasks. In order to study the possible ecological role of these materials further, an assay util


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 270 W. BELL AND R. MITCHELL medium; the gradual increase with age can be accounted for by evaporation of the medium in the flasks. It is evident that the bacteria tested are able to coexist in culture with these algae. The data suggest that most of these bacteria depend on degradation products of the algal cells. A small residual population of bacteria existed in a viable state throughout the 30-day period tested, even in control flasks. In order to study the possible ecological role of these materials further, an assay utilizing bacterial chemotaxis was developed and employed in a series of experi- ments with intentions similar to those reported above—, the determination of the effect of algal culture age on bacterial response. Controls for these experiments consisted of capillary tubes containing filtered F/2A of the same age as the algal culture being tested. The results of one such experiment are shown in Figure 3. The bacterium was isolate 7697, one of those used in the previous growth experiments. The chemo- taxis pattern obtained is typical and can profitably be compared with the growth data of Figure 2. Filtrates from young algal cultures did not attract the bacterium. 300 r- ISOLATE 8716 ISOLATE 8715 ISOLATE 8714. 20 30 MINUTES 40 50 FIGURE 4. Attraction of bacteria from enriched Vineyard Sound water to filtrate from 30-day Skelctonema culture. These bacteria, from the same water sample, could be readily differentiated on plates on the basis of colony morphology and color. No other bacteria appeared in significant numbers in this Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology