Annual report of the North Carolina Agricultural Experiment Station . n length. Slow lineal growth and much branchinggives many narrow zones; rapid lineal growth with infrequent branchingcauses broad zones. Sclerotinia Libertiana Fckl., from lettuce. Zonal sclerotial formation is exhibited by this fungus. (Fig. 8.) Thatthis phenomenon may be attributed to crowding of the mycelium is indicatedby the fact that adjacent colonies form more sclerotia at their points of con-tact. (Fig. 9.) ^olz, Ueber die Bedingungen der Entstehung der durch Sclerotinia fractigena der Aepfel.
Annual report of the North Carolina Agricultural Experiment Station . n length. Slow lineal growth and much branchinggives many narrow zones; rapid lineal growth with infrequent branchingcauses broad zones. Sclerotinia Libertiana Fckl., from lettuce. Zonal sclerotial formation is exhibited by this fungus. (Fig. 8.) Thatthis phenomenon may be attributed to crowding of the mycelium is indicatedby the fact that adjacent colonies form more sclerotia at their points of con-tact. (Fig. 9.) ^olz, Ueber die Bedingungen der Entstehung der durch Sclerotinia fractigena der Aepfel. Cent, f. Bak. II. Ab. 17, 175. Hutchinson, Ueber Form und Bau der Kalonieen niederer Pilze. Cent. f. Bak. II, Ab. 17,602. Also Hedgecock, Zonation in Artificial culture of Cephalothecium and other fungi. Ann. Rpt. 17,Mo. Bot. Card., 1906. 2Milburn, Ueber Aenderungen der Farben bei Pilzen und Bakterien. Cent. f. Bak. Ab. II,13, 257. 3Istvanffi, Etudes Microbiologiques et mvcologiques sur le rot gris de la vigne. Am. dellnstitut Cent. Ampel. Roy. Hongrois, 1905, Fig. 8.—Sclerotinia Libertiana Fckl., showing zonal formation of sclerotia on corn meal culture. it P\*\ *•/>* ****** 5*** 3. 9.—Sclerotinia Libertiana Fckl., showing the formation of sclerotia in greater abundance THIRTY-SECOND ANNUAL REPORT, 1009. SUMMARY REGARDING DENSITY OF MYCELIUM. Zone formation in Ascochyta Chrysanthemi is due to crowding of mycelium,not to light or heat relation. A similar conclusion was reached by Istvanffi(Istvanffi lc.) regarding the very striking zones shown by Hclerotinia. Thesame causes may apply also with Daldinia concent rica and many other fungiof similar structure. III. Chemical Relations. Chemical relations have been studied with eleven fungi, the fungus beingusually grown in agar with varying nutrients added. Occasionally othermedia were used. A chemical base agar (cba) was made of the followingproportion: Water 1,000 grams. Di-potassium phosphate grams. Calcium chl
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