. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CARDIOACTIVE FARPs IN LIML'LUS 313 A. Cardiac Ganglion Bursts Control Control 10"8 M SDRNFLRFa 10"8MTNRNFLRFa zi. o B. Time Course 1 sec 3 CQ o> 1/3 ea tu 80 H 60- 40- -£ 20- 1) u cu PH 0- D SDRNFLRFa • TNRNFLRFa. 0 1 5 10 i 15 20 25 3i Time (min) Figure 2. The chronotropic action of TNRNFLRFamide and SDRNFLRFamide (a = amide) on the Limulus heart is due to excitation of cardiac ganglion neurons. A. At 10"* M, these peptides increased the rate of bursting in isolated ganglia, while decreasing the interburst
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CARDIOACTIVE FARPs IN LIML'LUS 313 A. Cardiac Ganglion Bursts Control Control 10"8 M SDRNFLRFa 10"8MTNRNFLRFa zi. o B. Time Course 1 sec 3 CQ o> 1/3 ea tu 80 H 60- 40- -£ 20- 1) u cu PH 0- D SDRNFLRFa • TNRNFLRFa. 0 1 5 10 i 15 20 25 3i Time (min) Figure 2. The chronotropic action of TNRNFLRFamide and SDRNFLRFamide (a = amide) on the Limulus heart is due to excitation of cardiac ganglion neurons. A. At 10"* M, these peptides increased the rate of bursting in isolated ganglia, while decreasing the interburst interval and the duration of individual bursts. B. Time course of the effect of FaRPs on the isolated ganglion. The bar depicts the duration of peptide application. gested that FaRPs alter the excitability of cardiac muscle fibers. To test this hypothesis, we applied FaRPs to de- ganglionated muscle rings. Both TNRNFLRFamide and SDRNFLRFamide, at 3 X 1(T7 M, caused these prepa- rations to beat irregularly in the absence of neural or elec- trical stimulation (Fig. 4). Peptide-induced contractions were abolished with the addition of 20 mAI magnesium to the superfusion (data not shown). Partial characterization oj Limulus FaRPs Acidified methanol extracts of the Limulus prosomal CNS were partially purified by reverse phase HPLC in three consecutive protocols; the purification was moni- tored with the Busycon RPM bioassay. With a linear gra- dient of 2 to 60% acetonitrile with the C8 column, we detected two peaks of FaRP-like bioactivity. The sepa-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Claren
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
