. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figures 4-9. ImmunohistochemicaJ localization of vacuolar-type ATPase (V-ATPase) in whole accessory boring organ (ABO) of Nucellu lamellosa. Figure 4. This section was probed with polyclonal antibody to the V-ATPase d (39-kDa) subunit and alkaline-phosphatase-conjugated secondary antibody. Immunoreactive sites were visualized with BCIP/NBT. Immunostaining is exclusive to the brush border of the ABO (b). Immunostaining is absent in the foot epithelium (arrowheads), e, epithelial cells; s, sinus. Scale bar: 100 fim. Figure 5.
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figures 4-9. ImmunohistochemicaJ localization of vacuolar-type ATPase (V-ATPase) in whole accessory boring organ (ABO) of Nucellu lamellosa. Figure 4. This section was probed with polyclonal antibody to the V-ATPase d (39-kDa) subunit and alkaline-phosphatase-conjugated secondary antibody. Immunoreactive sites were visualized with BCIP/NBT. Immunostaining is exclusive to the brush border of the ABO (b). Immunostaining is absent in the foot epithelium (arrowheads), e, epithelial cells; s, sinus. Scale bar: 100 fim. Figure 5. Magnified image of the ABO shown in Figure 4. Note the positive reaction for V-ATPase in the brush border (b). e, epithelium; s, sinus. Scale bar: 75 /MUI. Figure 6. This section was probed with the anti-39kDa primary antibody (as above) and HRP-conjugated secondary antibody. Immunoreactive sites were visualized using DAB-HiO,. The imrnunostaining is exclusive to the brush border (b). e, epithelium. Scale bar: 40 Figure 7. This section was probed with monoclonal antibody to the V-ATPase B (60-kDa) subunit. primary antibody, and alkaline-phosphatase-conjugated secondary antibody. Immunoreactive sites were visualized with BCIP/NBT. Immunostaining is exclusive to the brush border of the ABO (b). Arrowheads, foot epithelium; e, epithelial cells; s, sinus. Scale bar: 100 jum. Figure 8. Magnified image of the ABO seen in Figure 7. Very intense imrnunostaining is noted in the brush border (b). e, epithelium. Scale bar: 25 /nm. Figure 9. ABO probed only with alkaline-phosphatase-conjugated secondary antibody and BCIP/NBT. No imrnunostaining is apparent in the brush border (b). e, epithelium. Scale bar: 25 /nm. tions incubated in anti-B subunit polyclonal antibody showed the same Immunostaining pattern. Both the anti-B subunit (Figs. 7 and 8) and and-A subunit monoclonal antibodies showed positive staining for V-ATPase within the brush border. Control sections incubated without a
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
