. Manual of bacteriology. Bacteriology. CULTIVATION OF THE GONOCOCCUS. 225 The young colonies are visible within forty-eight hours, and often within twenty-four hours. They appear around the points of inoculation as small serai-transparent discs of irregularly rounded shape, the margin being undulated and sometimes showing small processes. The colonies vary somewhat in size and tend to remain more or less separate. They generally reach their maximum size on the fourth or fifth day, and are usually found to be dead on the ninth day, often much earlier. On the medium of Wertheim the period of ac
. Manual of bacteriology. Bacteriology. CULTIVATION OF THE GONOCOCCUS. 225 The young colonies are visible within forty-eight hours, and often within twenty-four hours. They appear around the points of inoculation as small serai-transparent discs of irregularly rounded shape, the margin being undulated and sometimes showing small processes. The colonies vary somewhat in size and tend to remain more or less separate. They generally reach their maximum size on the fourth or fifth day, and are usually found to be dead on the ninth day, often much earlier. On the medium of Wertheim the period of active growth and the duration of life are somewhat longer. Even if impurities are present, pure sub-cultures can generally be obtained by the above methods from colonies of the gonococcus which may be lying separate. In the early stage of the disease the organism is present in the male urethra in practically pure condition, and if the meatus of the urethra be sterilised by wash- ing with weak solution of corrosive sublimate and then with absolute alcohol, and n\. the material for inoculation be expressed from the deeper part of the urethra, cultures may often be obtained which are pure from the first. By * *' â¢- successive sub-cultures at short intervals, growth may FlO. from a pure culture ' ^ -'on blood agar 01 twenty-four hours growth. be maintained indefinitely. Some already are beginning to show the swollen d,i â â â J 11 appearance common in older cultures. the org-anism gradually =, â j k i ,i â t,i !r) tt J Stained with carbol-thionin-blue. X loco. flourishes more luxuriantly. In culture the organisms have similar microscopic characters to those described (Fig. 86), but show a remarkable tendency to undergo degeneration, becoming swollen and of various sizes, and staining very irregularly. Degenerated forms are seen even on the second day, whilst in a culture four or five days old comparatively few normal cocci may be found. The less suitable the
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