. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . :101:201:40 1:5°1:80i: iqo1:2001:500I:1000I:2000I:5000I:I0000I:20000I:50000I:I00000I:200000 DILUTION TABLE FOR WATER OR MILK ANALYSIS Number Amount of Dilution Amount ofSterile Water Final Dilution I Original i: I 2 ICC. of No. I 1:100 3 of No. i! 1:500 4 of No. 2 I:1000 5 of No. 2 I:2000 6 of No. i! I:5000 7 ICC. of No. 2 I:I0000 8 of No. 4 I:20000 9 of No. 4 I:50000 10 ICC. of No. 4 I:I00000 II o
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners . :101:201:40 1:5°1:80i: iqo1:2001:500I:1000I:2000I:5000I:I0000I:20000I:50000I:I00000I:200000 DILUTION TABLE FOR WATER OR MILK ANALYSIS Number Amount of Dilution Amount ofSterile Water Final Dilution I Original i: I 2 ICC. of No. I 1:100 3 of No. i! 1:500 4 of No. 2 I:1000 5 of No. 2 I:2000 6 of No. i! I:5000 7 ICC. of No. 2 I:I0000 8 of No. 4 I:20000 9 of No. 4 I:50000 10 ICC. of No. 4 I:I00000 II of No. 7. I:200000 12 of No. 7. I:500000 13 ICC of No. 7 I:I000000 ii8 LABORATORY GUIDE IN BACTERIOLOGYEXERCISE II. BACTERIOLOGICAL EXAMINATION OF AIR An approximate determination of the number ofbacteria in the air can be made by the following simplemethod: Place a certain amount (50 ) of broth inan Erlenmeyer flask (Fig. 36, a). This flask is providedwith a rubber stopper (b), provided with two holes,through which the two glass tubes (c with a wide opening. —a ».> Fig. 36Apparatus for Determining the Number of Bacteria in a DefiniteVolume of Aira. Erlenmeyer flask /. Five-liter flask 6. Rubber stopper g-h. Glass tubes c-d. Glass tubes i. Pinchcock and d) lead. Cotton plugs are then inserted at c and d,and the apparatus is steriUzed in the autoclav. Alarge bottle (/), containing 5 liters of water, is thenprovided with a rubber stopper, and also with two glasstubes {g and h); h is connected with a short piece ofrubber hose and a pinchcock (j). When the Erlen- EXAMINATION OF WATER, AIR, AND MILK 119 meyer flask and contents are sterilized, the tube d is con-nected, by means of the rubber hose e, with g, and theplug at c is removed. By opening the pinchcock i, 5liters of air are aspirated through the broth in flask flask is then disconnected, and i. is plated inagar and i in gelatin. The former is incubated at 37,and the latter
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