. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. nM 10 nM 30 nM 50 nM 100 nM Calcium Added 500 nM iPLA2 Activity is Effected by Modulators of PFK Activity Condition Tested Control Citrate AMP ADP ADP * Creatine Phosphate AMP-PCP Mean Std Dev % of Mean F-6-P. fflflf[f[ff mm Favoring Conditions: • Presence of AMP • Presence of ADP • Low ATP / ADP Ratio Arachidonic Acid •Abundant ATP • Presence of Citrate • High ATP / ADP Ratio • AMP-PCP Figure 1. (A) A histogram .showing the calcium dependence of the s
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. nM 10 nM 30 nM 50 nM 100 nM Calcium Added 500 nM iPLA2 Activity is Effected by Modulators of PFK Activity Condition Tested Control Citrate AMP ADP ADP * Creatine Phosphate AMP-PCP Mean Std Dev % of Mean F-6-P. fflflf[f[ff mm Favoring Conditions: • Presence of AMP • Presence of ADP • Low ATP / ADP Ratio Arachidonic Acid •Abundant ATP • Presence of Citrate • High ATP / ADP Ratio • AMP-PCP Figure 1. (A) A histogram .showing the calcium dependence of the specific activities (absorbance/second/milligram protein) of phospholipase A2 ( associated with each of four membrane subfractions. Membrane subfractions A. B. C. and D sc<limcnl unto , and M sucrose, respectively. Note the calcium-Independent phospholipase A2 (iPLA^) activities (0 nM calcium), ami the peaks of calcium-dependent phospholipase A-, (cPLA2) activity ut JO unit 100 nM calcium. A minimum of triplicate assavs performed on ciftlil different pn-EK preparations; the mean values are presented. The standard deviations from the mean of PIA: <i< ti\'ii\. measured fur each subtraction ill cucli calcium concentration, did not exceed of the mean values. A ke\ to bars in the histogram hai i\ within the figure, itt) The effect upon iPLA, of modulator', of phosphofructokinase {PFK) activity. Modulators of PFK were present at a final concentration of I inM. The data presented represent the mean values for those the standard deviations were between ,„,(/ of those values. fC) A .schematic of the "metronome" model for reversible co-rcKiilalion of the iPLA? and PFK activities on the pn-EK of the prophase mitotic apparatus. hut the activity was again lower in subtractions A and C. In 300 nM calcium, PLA, activity declined in each subtraction relative to values for iPLA2 activity. Finally, in 500 nM calciu
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
