Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . any years investigation, that he found specific bacil-lus in all leprous processes. Form.—SmaU slender rods,somewhat shorter than tu-bercle bacilh, otherwise verysimilar in in the form norstaining reactions can be distinguished fromB. tuberculosis. In the interior of the celltwo or three oval spaces areusually seen, not believed tobe spores. They are — Bordoni-Uffred-uzzi have obtained growthsupon blood-serum to which peptone
Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . any years investigation, that he found specific bacil-lus in all leprous processes. Form.—SmaU slender rods,somewhat shorter than tu-bercle bacilh, otherwise verysimilar in in the form norstaining reactions can be distinguished fromB. tuberculosis. In the interior of the celltwo or three oval spaces areusually seen, not believed tobe spores. They are — Bordoni-Uffred-uzzi have obtained growthsupon blood-serum to which peptone and glycerin had beenadded, but the accuracy of this observation was doubted,and not imtil Clegg, in 1909, and Duval, in 1910, in work inthe Philippine Islands de\ised special media was it possibleto obtain readily initial and subcultures. The method depends upon supplying the organism withalbumin partially metabolized. Clegg prepared this byplanting the lepra bacilh on media containing ameba andbacteria; then, by short sterilization, destroying these, whilethe resistant B. lepra hved on. Duval, by adding trypsin to. Fig. 51.—Pure culture of bacillus of leprosy, showing the charaCteristic morphology and arrange-ment of the bacilh (Duval). BACILLUS TUBERCULOSIS AND ALLIED ORGANISMS 123 egg-albumin or blood-serum, was able to change the proteinsufficiently without reqmring ameba or bacterial digestion. The leprous nodule is cut in small slices and spread overan albumin slant or Petri plate, and the surface covered withI per cent, solution trypsin and placed in oven at 37° C. forten days. The growth is moist and becomes yellow after severalgenerations; it is on surface. Staining.—B. lepra resist the decolorizing action of acids,as the tubercle baciUi, but they are more easily stained, re-quiring but a few minutes more with the ordinary watery solu-tions. They take Grams stain readily. Pathogenesis.—Arning inoculated a prisoner with tissueobtained from leprous patients
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