. The Biochemical journal. Biochemistry. 282 A. HOMER rt'tniirt'd lor tlicii' prcciititat idii lioiii their it'spcct ivc .scpjiratc .solutions in saline; then' was also a more marked overlappin;^ f the j)recipi(ation limits for tlie individual ]>roteins than was found for theii' separate solutions. Thus, fiom solution in normal saline, the Indk ol the iiscudoulohulin is preci])itated between the limits of .'^2 and 50 per cent, of saturation with ammonium sulphate, and ])etween 13 and 23 per cent, of anhydrous sodium sulphate: the alhumin under similai' conditions is mainly precipitated betw
. The Biochemical journal. Biochemistry. 282 A. HOMER rt'tniirt'd lor tlicii' prcciititat idii lioiii their it'spcct ivc .scpjiratc .solutions in saline; then' was also a more marked overlappin;^ f the j)recipi(ation limits for tlie individual ]>roteins than was found for theii' separate solutions. Thus, fiom solution in normal saline, the Indk ol the iiscudoulohulin is preci])itated between the limits of .'^2 and 50 per cent, of saturation with ammonium sulphate, and ])etween 13 and 23 per cent, of anhydrous sodium sulphate: the alhumin under similai' conditions is mainly precipitated between T)! and 7(1 per cent, of saturation with amiiioniuiii sulpliate and between 2<» and .") I per cent, of sodium sulphate. Vi' :?. The precipitation of plasma A (adjustod to Ph8-8) by ammonium sulpliate - 10 B'. 22 24 26 28 30 32 34 36 38 40 42 44 46 48 50 Percentages of saturation with ammonium sulphate The continuous Curve I represents the precipitation of the unheated plasma A (Ph8-8) The intermittent Curve II represents the precijiitation of the plasma A (Pjj8-8) aftei it had been heated to 57-5^ C. for 5 hours On the other hand there was an appreciable precipitation of pseudo- globulin from unheated unadjusted plasma (Pjj74) on the addition of ammonium sulphate to the extent of 28 per cent, of saturation or of 11-5 per cent, of anhydrous sodium sulphate. Moreover, while the precipitation of the pseudoglobulin from the plasma was complete at 46 per cent, of saturation with ammonium sulphate or on the addition of 18-5 per cent, of sodium sulphate, there was, even at these concentrations of the sulphates, an appre- ciable precipitation of albumin. The precipitation of the latter from the plasma was complete at 67-5 per cent, of saturation with ammonium sulphate or at a concentration of 32 per cent, of anhydrous sodium Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration
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