. Biological structure and function; proceedings. Biochemistry; Cytology. 59° PETER MITCHELL TABLE IV Glucose 6-phosphatase Activity of Normal and Treated Escherichia coli (ML 30) Suspensions and of Growth and Suspension Media Glucose 6-phosphatase 0/ /O Activity Material activity of normal (/xmole P/g. min.) cells Normal intact cells 2-39 100 Benzene-treated cells 2-77 116 Frozen and thawed cells 2-19 92 Suspension mediuni oil 4-6 Growth medium 0-07 2-9 Normal intact cells (/xmole P/g. min. liberated in suspension medium only) 2-91 suspension medium. As illustrated in Fig. i, these and other
. Biological structure and function; proceedings. Biochemistry; Cytology. 59° PETER MITCHELL TABLE IV Glucose 6-phosphatase Activity of Normal and Treated Escherichia coli (ML 30) Suspensions and of Growth and Suspension Media Glucose 6-phosphatase 0/ /O Activity Material activity of normal (/xmole P/g. min.) cells Normal intact cells 2-39 100 Benzene-treated cells 2-77 116 Frozen and thawed cells 2-19 92 Suspension mediuni oil 4-6 Growth medium 0-07 2-9 Normal intact cells (/xmole P/g. min. liberated in suspension medium only) 2-91 suspension medium. As illustrated in Fig. i, these and other confirmatory observations forced us to the conclusion that the glucose-6-phosphatase of intact Escherichia coli is enclosed in a region between the cell wall and the surface of the osmotic barrier component of the plasma membrane which Cell wall (molecular sieve) I Plasma membrane ; Periplasm ''(osmotic barrier) Medium. Endoplasm Glucose-6-phosphate Fig. I. Diagram of cell wall, periplasm and plasma membrane (osmotic barrier component) in Escherichia coli. The glucose-6-phosphatase, partly adsorbed on a substratum in the cell envelope complex, is confined to the periplasm by the molecular sieve function of the cell wall. we might appropriately call the "periplasm". You may ask how one can show that the effective pore size of the cell wall of living Escherichia coli is small enough to prevent the passage of proteins between the periplasm and the external medium. Figure 2{a) shows living Escherichia coli (strain ML 30) in which the protoplasts have been made to retract from the cell wall by the addition of 0-4 M NaCl to a suspension medium of 0-02 M sodium phosphate buffer at pH 7, and Fig. 2(6) shows the same with the. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium
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