. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. RHODOPSIN IN ASTEROIDS AND ECHINOIDS 103. Figure 5. Digitized images of ahoral views of 2-3 segments of arms of Ophioderma brevispinum ob- tained by overlaying 40 con focal images taken at focal depth intervals of 10/jm. Each segment has two sets of 7 lateral spines each (not all spines \isible). (A) Control with dark-adapted animal incubated with anti- body buffer followed by secondary antibody. (B) Dark-adapted animal incubated with B6-30N antibody followed by secondary antibody. (C) Light-adapted animal incubated with B6-
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. RHODOPSIN IN ASTEROIDS AND ECHINOIDS 103. Figure 5. Digitized images of ahoral views of 2-3 segments of arms of Ophioderma brevispinum ob- tained by overlaying 40 con focal images taken at focal depth intervals of 10/jm. Each segment has two sets of 7 lateral spines each (not all spines \isible). (A) Control with dark-adapted animal incubated with anti- body buffer followed by secondary antibody. (B) Dark-adapted animal incubated with B6-30N antibody followed by secondary antibody. (C) Light-adapted animal incubated with B6-30N antibody followed by secondary antibody. Brightness and contrast in A. B. and C are identical. Scale bar is 200 pm. (D) Digiti/ed image of a single longitudinal section of the tip of the central arm spine in B. Scale bar is 10 /am. SP. arm spine: A. aboral arm plate; C. cuticle: E, epidermis; D, dermis. structural investigations have shown that these micro- villi and retinal proteins are more abundant in dark- adapted animals than in light-adapted animals (Takasu and Yoshida, 1983). Therefore, the substance in the lu- men of the optic cups that cross-reacts with the two monoclonal antibodies is probably a rhodopsin and thus, given the specificity of the antibodies in these animals, the cross-reacting protein found in the Western blots. The possibility remains, however, that the two mono- clonal antibodies are reacting specifically with a protein that is unrelated to photoreception. There are two argu- ments against this possibility. The first is based on the biochemical characteristics of the cross-reacting pro- tein—if the protein is not rhodopsin, it is a membrane- associated protein similar in size to vertebrate rhodop- sins. The second involves location—the immunochem- istry suggests that the cross-reacting protein is strongly concentrated in the optic cups of A. Jorbesi and more common in dark-adapted than light-adapted animals, suggesting that the protein is invol
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