. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. % hairs stripped per one setal segment Figure 3. Distribution of the number of segments in which the per- centage of the ovigerous hairs that slipped out of the coat without damage was estimated, (a) Data from experiments in which the tip of the seta remained (incubation in 10 ppt SW). (b) Data from experiments in which the seta was cut at its tip as shown in Figure la (incubation with 10 ppt SW). (c) Embryo clusters treated with hatch water. N = Total number of subdivided setal segments with their attached egg clusters. The
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. % hairs stripped per one setal segment Figure 3. Distribution of the number of segments in which the per- centage of the ovigerous hairs that slipped out of the coat without damage was estimated, (a) Data from experiments in which the tip of the seta remained (incubation in 10 ppt SW). (b) Data from experiments in which the seta was cut at its tip as shown in Figure la (incubation with 10 ppt SW). (c) Embryo clusters treated with hatch water. N = Total number of subdivided setal segments with their attached egg clusters. The per- centage for each setal segment is shown at intervals of KK. hairs certainly tended to slip out most easily. This ten- dency was especially marked at the tip of the seta (Fig. 3a). So in most of the experiments, the tip of the ovigerous seta was cut away before use (arrow in Fig. Ib). On av- erage, only 10% of the hairs were stripped clean without damage (Fig. 3b). In contrast, about 80% of the egg clusters slipped off when the preparation was placed into hatch water (Fig. 3c). A further difficulty is that the response to a given con- centration of OHSS is considerably different even among the egg clusters produced by the same female. Figure 4 shows the response of two groups of the egg clusters that were separated from one female and both immersed in hatch water with the same concentration of OHSS. Al- though the percentage of stripped hairs fluctuated consid- erably, it clearly increased with time, reaching a maximum in 1-3 h ( Fig. 4). In contrast, control egg clusters immersed in 10% seawater showed no such increase in the percentage of stripping. The fluctuation in the re- sponses suggests that the adhesion between the investing coat and the ovigerous hairs is also variable, even in the same female. Concentration-dependence of OHSS activity Unhatched embryos detached from two females of S. haematocheir were incubated with a series of threefold dilutions of hatch
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
