. The clinical pathology of the blood of domesticated animals. Blood; Veterinary medicine. HISTOLOGICAL EXAMINATION 17 thoroughly clean. Unused slides may be cleaned in strong soap or ''gold dust" solution, well rinsed in water, then placed in al- cohol from which they are wiped and polished. Slides with ground edges are preferred. The edge of a slide is touched to the top of a fresh drop of blood then applied to another slide at an angle o f about forty degrees (Fig. 10). As soon as the blood has spread along the line of contact of the two slides, t h e smearer is drawn ^ig. 10. Spreadin
. The clinical pathology of the blood of domesticated animals. Blood; Veterinary medicine. HISTOLOGICAL EXAMINATION 17 thoroughly clean. Unused slides may be cleaned in strong soap or ''gold dust" solution, well rinsed in water, then placed in al- cohol from which they are wiped and polished. Slides with ground edges are preferred. The edge of a slide is touched to the top of a fresh drop of blood then applied to another slide at an angle o f about forty degrees (Fig. 10). As soon as the blood has spread along the line of contact of the two slides, t h e smearer is drawn ^ig. 10. Spreading film on glass slide, along with very gentle pressure slowly leaving a thin smear of blood on the other slide. The smear should cover one-half or two-thirds of the slide. A little practice will enable one to make good smears at each attempt. The smears may be kept for some time without altering their staining properties if they are kept dry. After several weeks, however, not so good results can be obtained from certain of the more delicate stains, Jenner's, Wright's and Hasting's. Fixation and staining. Jenner's stain is one of the most rapid and easy to manipulate of the many methods in use and stains each of the several kinds of granules in the leucocytes. It is rec- ommended for ordinary examinations. The staining fluid is a five-tenths per cent, solution of the dye (Gruebler's) in pure methyl alcohol (Merck's). This acts as both fixing and staining fluid. The smear, previously unfixed, simply dried in the air, is flooded with the staining fluid which is allowed to act two or three minutes, when it is washed in distilled water until the better spread portions have a pinkish tint, which usually requires about ten seconds. The water should then be shaken and blown vigorously from the specimen which is then dried rapidly in the air. As soon as it is thoroughly dry it may be examined using a two mm. (~ in.) oil immersion objective. It is not necessary to place a cover glass on
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectblood, bookyear1917
