. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 96 H. C. BOYD ET AL. 40 # »••••* lx. 15°C o o 2x. 15°C 2 +— -f lx. 20° C 0 30 • « 2x. 20° C 0 I D S 20 - C 0 n 10 . 0 N Y 1 *»r^" •t- * * 4 $ ^^ I °0 20 40 60 80 DAYS 260 200 140 80 40 30 20 10 •••••• lx. 15°C o o 2x. ° C *--» lx. 20°C »— • 2x. 20° C A—4 Marina B. 20 40 DAYS 60 80 FIGURE 1. Botryllus growth curves for Phase I. The ordinate represents mean number of zooids per colony. Day 0 = 4/23/84. Note that IX, 2X, etc. refer to the food concentrations, and not the frequency of daily feeding. (Zooid counts were no
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 96 H. C. BOYD ET AL. 40 # »••••* lx. 15°C o o 2x. 15°C 2 +— -f lx. 20° C 0 30 • « 2x. 20° C 0 I D S 20 - C 0 n 10 . 0 N Y 1 *»r^" •t- * * 4 $ ^^ I °0 20 40 60 80 DAYS 260 200 140 80 40 30 20 10 •••••• lx. 15°C o o 2x. ° C *--» lx. 20°C »— • 2x. 20° C A—4 Marina B. 20 40 DAYS 60 80 FIGURE 1. Botryllus growth curves for Phase I. The ordinate represents mean number of zooids per colony. Day 0 = 4/23/84. Note that IX, 2X, etc. refer to the food concentrations, and not the frequency of daily feeding. (Zooid counts were not continued for marina colonies after day 57 because the colonies were very large, such that in some cases the growing edges of two colonies on a slide had made contact, sometimes resulting in vascular fusion.) A: Colonies fed IX or 2X algae at 15°C and 20°C. B: Colonies fed IX or 2x Liquifry at 15°C and 20°C, or grown in Monterey Marina. 57; by day 78, approximately 70% of the IX Liquifry colonies and nearly 60% of the 2X Liquifry colonies were sexually mature. In the marina, eggs and/or embryos were first observed on day 50; by day 78, only about 40% of the marina colonies were sexually mature. Phase II: Healthy, rapidly growing Botryllus colonies were raised under longterm controlled laboratory conditions Although Botryllus colonies grow well in Monterey Marina from April to July (Phase I), we suspected that this would not be the case during winter months. To investigate this further, we initiated Phase II culture treatments in December, and chose higher temperature and food levels for the laboratory conditions based on the results from the Phase I experiment. Our major finding was that Botryllus colonies raised on a Liquifry diet at 20°C and 24°C in the laboratory grew faster than colonies grown in the marina in winter. In addition to the initial growth increase experienced by all colonies, those grown at the higher food l
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
