. Pathogenic micro-organisms, including bacteria and Protozoa; a practical manual for students, physicians and health officers. arts. No. 2. Bismarck brown 1 part. Boiling distilled water oOO parts. The Neisser stain has been advocated in order to separate the viru-lent from the non-virulent l^acilli, without the deUiy of inoculating 198 PA THOGENIC MICRO-ORGA NISMS. animals; but in our hands, with a very large experience, neither theNeisser stain nor other stains, such as the modifications of the Rouxstain, have given much more information as to the virulence of thebacilli than the usual meth
. Pathogenic micro-organisms, including bacteria and Protozoa; a practical manual for students, physicians and health officers. arts. No. 2. Bismarck brown 1 part. Boiling distilled water oOO parts. The Neisser stain has been advocated in order to separate the viru-lent from the non-virulent l^acilli, without the deUiy of inoculating 198 PA THOGENIC MICRO-ORGA NISMS. animals; but in our hands, with a very large experience, neither theNeisser stain nor other stains, such as the modifications of the Rouxstain, have given much more information as to the virulence of thebacilli than the usual methylene-blue solution of Loeffler. A fewstrains of virulent bacilli fail to show a marked characteristic stain,and quite a few pseudodiphtheria bacilli show the dark bodies. Thereare also in many throats bacilli which seem to have all the staining Fig. 81 Fig. 82 Fig. 81.— B. diphtheria agar culture. Bacilli small and uniform in shape. X 1000 82.—B. diphtheria. Forty-eight hours agar culture. Thick, Indian-clubbed rods andmoderate number of segments. One year on artificial culture media. X 1410 Fig. S3 Fig. 84 4^ ^. s. ^ %K^ /
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