. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 142 M. R. ELPHICK ET AL was embedded by routine methods in paraffin wax (58°C mp), sectioned at 7-15 ^m, and mounted on poly-L-ly- sine coated glass slides. The primary rabbit antisera to FMRFamide (1171 from C. J. P. Grimmelikhuijzen, and LI35 from G. J. Dockray) were applied at dilutions be- tween 1:100 and 1:1000. The 1171 antiserum has been characterized by solid and liquid phase absorption tests with numerous, potentially cross-reactive peptides; it has high affinity for FMRFamide, as expected, but also some affinity fo
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 142 M. R. ELPHICK ET AL was embedded by routine methods in paraffin wax (58°C mp), sectioned at 7-15 ^m, and mounted on poly-L-ly- sine coated glass slides. The primary rabbit antisera to FMRFamide (1171 from C. J. P. Grimmelikhuijzen, and LI35 from G. J. Dockray) were applied at dilutions be- tween 1:100 and 1:1000. The 1171 antiserum has been characterized by solid and liquid phase absorption tests with numerous, potentially cross-reactive peptides; it has high affinity for FMRFamide, as expected, but also some affinity for FLRFamide, FMKFamide, LTRPRYamide and RFamide (Grimmelikhuijzen, 1984). Two methods were used to visualize the bound pri- mary antibodies: application of a fluorescein isothiocya- nate (FITC)-labelled swine anti-rabbit second antibody; or treatment with a peroxidase conjugated goat anti-rab- bit second antibody, followed by rabbit peroxidase anti- peroxidase (PAP) complex and diaminobenzidine as the peroxidase substrate (the PAP method). Three controls were carried out: the primary antibod- ies were pre-absorbed overnight at room temperature with 10 nmol of FMRFamide per ml of diluted antise- rum; antibodies to other peptides (anti-insulin, anti-sub- stance P, anti-cholecystokinin) were tested; and non-im- mune serum was also examined. Results General morphology The major components of the starfish nervous system are the circumoral nerve ring and its five branches—the radial nerve cords—which extend along the ventral sur- face of each arm (Fig. la). These nerve tracts contain two distinct parts, the ectoneural and hyponeural systems (Fig. 1 b). The ectoneural system is further organized into an outer epithelial region containing cell bodies and sup- porting cells, and an inner axonal region traversed by fi- bers from the supporting cells (Fig. Ib). It is continuous with an extensive sub-epithelial nerve plexus of the skin, which is thickened locally to form the marginal
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
