. Biological structure and function; proceedings. Biochemistry; Cytology. 6o GERTRUDE E. PERLMANN TABLE I Amino Acid Composition of Crystalline Pepsin [4] Nature of amino acid No. of residues per molecule Per cent of total Acidic: (Asp,Glu) . 71 20-7 Basic: (His,Lys,Arg) 4 1-2 Non-polar: (,Leu,lieu ,Ala,Met) 137 40-0 Hydroxy: (Ser,Thr) 72 20-9 Aromatic: (Tyr,Try,Phe) 38 II 'I Proline .... IS 4-4 1/2 Cys (-S-S-) . 6 1-7 Total 343 lOO-O That pepsin is a tightly folded molecule is indicated by its low intrinsic viscosity. The question arose, therefore, as to whether or not reagents could b
. Biological structure and function; proceedings. Biochemistry; Cytology. 6o GERTRUDE E. PERLMANN TABLE I Amino Acid Composition of Crystalline Pepsin [4] Nature of amino acid No. of residues per molecule Per cent of total Acidic: (Asp,Glu) . 71 20-7 Basic: (His,Lys,Arg) 4 1-2 Non-polar: (,Leu,lieu ,Ala,Met) 137 40-0 Hydroxy: (Ser,Thr) 72 20-9 Aromatic: (Tyr,Try,Phe) 38 II 'I Proline .... IS 4-4 1/2 Cys (-S-S-) . 6 1-7 Total 343 lOO-O That pepsin is a tightly folded molecule is indicated by its low intrinsic viscosity. The question arose, therefore, as to whether or not reagents could be found that would unfold the polypeptide chain and if unfolding 100 80 60- ^ 40 20. ⢠Dilute HC o â¢â â ⢠+ 8m urea A â ⢠â ⢠+ 3m guanidine hydrochloride 0 1-0 20 30 40 5-0 pH Fig. I. pH dependence of the activity of pepsin for the hydrolysis of haemo- globin. occurred, how certain properties, such as viscosity, optical rotation and the biological activity of the enzyme, would be affected. The first two reagents which were investigated for their effect on pepsin were urea and guanidine hydrochloride which are known to have a pro- found influence on the biological activity of enzymes, ribonuclease [5], chymotrypsin [6] and trypsin [7]. In contrast to the enzymes, which are. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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