. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 350. Figure 3. Summary sketches of developing ganglia of cephalic plate and visceral loop in Mtiihc lamina; postero-lateral views from right side. A. newly hatched larva. B. larva at mantle retraction stage. C. late larval stage. D. metamorphic stage. CC = cerebral commissure; CG = cerebral ganglion; EY = eye: LPP = left pallial placode; OS = osphradial neurons; RG = rhinophoral ganglion; SBG = subintestinal ganglion; SPG = su- praintestinal ganglion; ST = statocyst; VG = visceral ganglion; VL = visceral loop: VP = visceral
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 350. Figure 3. Summary sketches of developing ganglia of cephalic plate and visceral loop in Mtiihc lamina; postero-lateral views from right side. A. newly hatched larva. B. larva at mantle retraction stage. C. late larval stage. D. metamorphic stage. CC = cerebral commissure; CG = cerebral ganglion; EY = eye: LPP = left pallial placode; OS = osphradial neurons; RG = rhinophoral ganglion; SBG = subintestinal ganglion; SPG = su- praintestinal ganglion; ST = statocyst; VG = visceral ganglion; VL = visceral loop: VP = visceral placode. Larvae that hatched from egg masses laid in the field or laboratory were reared according to the method of Bickell and Kempf (1983). Under laboratory conditions and a rearing temperature of 12 to 14°C, larvae required a min- imum of 5 weeks to complete pre-metamorphic devel- opment. Larvae were anaesthetized as described by Bickell and Kempf (1983), and were fixed according to the method of Bickell and Chia( 1979). The area of the larval body containing developing gan- glia was thin sectioned in whole or part with a diamond knife, and batches of eight to ten sections were collected on uncoated copper grids (150 mesh size). The grids were first washed in acetone and distilled water, then passed briefly through the flame of an alcohol burner; this eases the pick-up of floating sections, possibly by reducing the hydrophobicity of the grid surface. To discourage the ten- dency of sections to float towards the grid periphery, grids were bent slightly so that sections were lifted onto a convex grid surface without excess water. The central areas of wet sections were teased over openings between grid bars with an eyebrow hair mounted on an orange stick. With this method, two to six whole sections per grid could be viewed, with each gridload of sections representing ap- proximately of tissue thickness. Sections were stained for 90 min in aqueous 2% uranyl acetate and
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
