. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. EFFECTS OF CATIONS ON NEMATOCYSTS 99 microtubes and centrifuged at 1940 X g for 5 min. One ml of each test solution was added to the pellet. Nema- tocysts were resuspended in the test solutions and allowed to stand for 10 min. Test solutions were ASW and 1 M solutions of NaCl, KG, CaCl:, MgCl2, and SrCl2. Next, the nematocysts were washed in DW by centrifuging at 1940 X g for 5 min and by resuspending the nematocysts in 1 ml of DW. The nematocysts were washed in DW and collected by centrifugation three times. Finally, the ne
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. EFFECTS OF CATIONS ON NEMATOCYSTS 99 microtubes and centrifuged at 1940 X g for 5 min. One ml of each test solution was added to the pellet. Nema- tocysts were resuspended in the test solutions and allowed to stand for 10 min. Test solutions were ASW and 1 M solutions of NaCl, KG, CaCl:, MgCl2, and SrCl2. Next, the nematocysts were washed in DW by centrifuging at 1940 X g for 5 min and by resuspending the nematocysts in 1 ml of DW. The nematocysts were washed in DW and collected by centrifugation three times. Finally, the nematocysts were resuspended in 150 n\ of DW. Aliquots (20 n\) of the nematocyst suspension were placed on meshes with formval or collodion membranes that had been coated with carbon and treated with poly-1-lysine. The nematocysts were allowed to settle on the membrane for 1 h, then air-dried after the remaining solution was soaked up with a piece of filter paper. Specimens were observed under a scanning transmission electron micro- scope (JEOL JEM-2000EX) equipped with an energy dis- persive spectrometer (TN 42 U). X-ray spectra were ac- quired at an acceleration voltage of 100 kV. Semiquan- titative elemental analyses were performed using an application software (Noran Instruments Inc. SMTP) on 4-6 nematocysts for each test solution. The software, which was designed for standardless semiquantitative analysis of metallurgical thin films, removes background and integrates peak areas. The peak intensities were con- verted to ratios of element concentrations by multiplying by calculated K-factors. Correction for absorption was not made. The ASW contained (in mAI): NaCl, 480; KC1, 10; CaCl2, 10; MgCl2, 26; MgSO4, 29; and was adjusted to pH with 10 mM HEPES. All the salt solutions and DW were buffered to pH with 10 mM HEPES, and all the experiments were done at room temperature (23-26°C). Results Nematocysts isolated from acontia of Calliaclis polypus in ASW swelled in conce
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
