. Pathological technique; a practical manual for workers in pathological histology and bacteriology. nimals and with different cul-tures. They are best developed in animals which surviveabout three days. In animals which succumb after eighteenhours no macroscopic change may be apparent. On micro-scopical examination of the kidneys, however, small areas ofnecrosis will usually be found, mainly in the pyramids, sur-rounding masses of cocci. In the kidneys of animals whichsurvive longer all the grades of invasion of these necroticareas by leucocytes, up to regular abscess-formation, may betraced.
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. nimals and with different cul-tures. They are best developed in animals which surviveabout three days. In animals which succumb after eighteenhours no macroscopic change may be apparent. On micro-scopical examination of the kidneys, however, small areas ofnecrosis will usually be found, mainly in the pyramids, sur-rounding masses of cocci. In the kidneys of animals whichsurvive longer all the grades of invasion of these necroticareas by leucocytes, up to regular abscess-formation, may betraced. By cultures the organism will be found in large PATHOGENIC BACTERIA AND FUNGI. 255 numbers in the kidneys and urine of the rapidly fatal cases;and in smaller numbers in the other organs and blood of theheart. Occurrence.—The staphylococcus pyogenes aureus is foundmost commonly in pus-formations of a circumscribed cha-racter and also in a large number of pathological conditions,of which only the more important will be mentioned here. These are as follows: Osteomyelitis, peritonitis, pleuritis,. Fig. st. -Staphylococcus pyogenes aureus: stab-culture three days old in gela-tin (Frankel and Pfeiffer). endocarditis, meningitis, broncho-pneumonia, and puerperalsepticemia. It may also be found in the blood of the variousinternal organs at autopsies in cases in which a suppurativeor other acute inflammatory process is present anywhere,with or without metastatic abscess-formation. The organismalso occurs frequently in the dust of places inhabited byman, as well as on the surface of the skin and of the mu-cous membranes of the nose and mouth. 256 PATHOLOGICAL TECHNIQUE. Diagnosis.—The staphylococcus pyogenes aureus cannotusually be identified with any certainty by the cover-glassexamination alone. Cultures are necessary in order to dif-ferentiate from the other staphylococci and from the strep-tococcus. For practical purposes the identification of the pyogeniccocci may be made by th
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