. Circular. Agriculture; Agriculture -- United States. 30 CIRCULAR NO. 118, BUREAU OF PLANT INDUSTRY. in order to insure an even distribution of the flocculent cellulose. Tubes of this character, inoculated upon the surface with the spores or mycelium of some cellulose-dissolving fungus and kept from 20 to 40 days in a moist atmosphere at a tem- perature of 30° C, show a cleared zone in the upper layers. The hyphse of the fungus usually pene- trate the medium for only a few millime- ters of the culture medium, as shown in figure 1. A tube in this condition may be scratched with a file or diamo
. Circular. Agriculture; Agriculture -- United States. 30 CIRCULAR NO. 118, BUREAU OF PLANT INDUSTRY. in order to insure an even distribution of the flocculent cellulose. Tubes of this character, inoculated upon the surface with the spores or mycelium of some cellulose-dissolving fungus and kept from 20 to 40 days in a moist atmosphere at a tem- perature of 30° C, show a cleared zone in the upper layers. The hyphse of the fungus usually pene- trate the medium for only a few millime- ters of the culture medium, as shown in figure 1. A tube in this condition may be scratched with a file or diamond glass cutter near the bot- tom at the point where it must later be broken; the lower half of the tube is dipped in a disin- fecting solution, such as per cent mer- curic chlorid, and wiped dry with sterile filter paper. The bottom of the tube is cracked en- tirely around and re- moved by means of sterile forceps; the cotton plug in the mouth of the tube is pushed down the tube against the cul- ture, and the entire plug of agar is forced sufficiently beyond the broken rim so that by means of a sterile knife several sterile sections may be cut and trans- ferred immediately to the surface of a previously cooled layer of cellulose agar in a Petri dish. The cytase will diffuse slowly from [Cir. 118.]. Fig. 1.—Culture tubes showing the action of Penicillium pinophilum when growing upon media containing amorphous cellulose. At the left a sterile tube of cellulose medium is shown for Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original United States. Bureau of Plant Industry. Washington, D. C. : U. S. Dept. of Agriculture, Bureau of Plant Industry
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