. Fungous diseases of plants : with chapters on physiology, culture methods and technique . Fungi in agriculture. 18 CULTURE METHODS AND TECHNIQUE quantities of media should be sterilized three times. Between the periods of sterilization media should be placed at a temperature favorable for most bacterial development, and not in a cold place, this being in order that any spores might pass into the vegetative. Fig. 50. Autoclave, Erect Type, Clamped Top, heated by Gas (Photograph by Geo. M. Reed) state within twenty-four hours, and thus be killed at the next sterilization. A suggestion which ha
. Fungous diseases of plants : with chapters on physiology, culture methods and technique . Fungi in agriculture. 18 CULTURE METHODS AND TECHNIQUE quantities of media should be sterilized three times. Between the periods of sterilization media should be placed at a temperature favorable for most bacterial development, and not in a cold place, this being in order that any spores might pass into the vegetative. Fig. 50. Autoclave, Erect Type, Clamped Top, heated by Gas (Photograph by Geo. M. Reed) state within twenty-four hours, and thus be killed at the next sterilization. A suggestion which has been made by Theobald Smith is of interest in connection with the sterilization of media which may con- tain anaerobic bacteria. If such media are sterilized in thin layers, oxygen may have comparatively free access to any submerged. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Duggar, Benjamin M. (Benjamin Minge), 1872-1956. Boston ; New York : Ginn
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