. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 408 J. H. WELSH AND H. H. HASKIN most cases larger amounts caused a number of legs to be dropped during the injection process, or shortly after the injection was made. The legs dropped were almost always on the side on which the injections were In 20 per cent of the animals the injection of 25 gamma of acetylcholine caused one to three legs to be dropped without further stimulation. Fifty animals, each of which was injected with 25 gamma of acetyl- choline and its legs stimulated approximately five minutes after injec


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 408 J. H. WELSH AND H. H. HASKIN most cases larger amounts caused a number of legs to be dropped during the injection process, or shortly after the injection was made. The legs dropped were almost always on the side on which the injections were In 20 per cent of the animals the injection of 25 gamma of acetylcholine caused one to three legs to be dropped without further stimulation. Fifty animals, each of which was injected with 25 gamma of acetyl- choline and its legs stimulated approximately five minutes after injection, yielded results which are shown in Fig. 2. The first leg to be grasped too o K- o Ul o <t UJ a. 50 ACETYLCHOLINE. LEGS STIMULATED FIG. 2. Percentage autotomy in 50 animals each of which received 25 gamma of acetylcholine chloride five minutes before stimulation of the legs. The dashed line in this and the following figures is the " normal curve " of autotomy. was dropped in 98 per cent of all the animals, but immediately an " in- hibitory " phase appeared and the entire curve resembles the inhibitory part of the " normal ; It is apparent that the injection of acetyl- choline markedly affects the autotomizing process and, at this concentra- tion, eliminates the period of increasing tendency to autotomize. Eserinc.—Pctrolisthes was found to be remarkably sensitive to ese- rine. The injection of gamma or more caused, after a delay of one 3 The injection of 100 gamma of acetylcholine into each of six Pachygrapsits transversus (Gibbes), a rock crab, caused the autotomy of three to five legs, on the side of the injection, during the injection process. In two cases all of the legs on the opposite side were autotomized within a few seconds after Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology