. dan bai zhi zu xue cong xu lie dao gong neng. botany. 第ä¹ç« ç¶é¢ï¼èªå¨åèç½è´¨ç»åæçå®æ´éå¾ 163 Genomic Solutions, Inc. DERPPRO Serial* 2032. 10/21 masses malcheil (47*) pTMcin Molecular wtight/pl Spccio ECOLI P02418 Swiss Proi Accession No. 50S KIBOSOMAI, Protein name Mass matched Delia ppm PcptiJc MXjucncc 768 (R)LPNGVI,R(T) (K)VIVNVVAE(-) (R)NFLVPQGK(A) (K)NIEFFEAR(R) (K)LAEVLAAANAR(A) n (K)NIEFFEARR(A) 1
. dan bai zhi zu xue cong xu lie dao gong neng. botany. 第ä¹ç« ç¶é¢ï¼èªå¨åèç½è´¨ç»åæçå®æ´éå¾ 163 Genomic Solutions, Inc. DERPPRO Serial* 2032. 10/21 masses malcheil (47*) pTMcin Molecular wtight/pl Spccio ECOLI P02418 Swiss Proi Accession No. 50S KIBOSOMAI, Protein name Mass matched Delia ppm PcptiJc MXjucncc 768 (R)LPNGVI,R(T) (K)VIVNVVAE(-) (R)NFLVPQGK(A) (K)NIEFFEAR(R) (K)LAEVLAAANAR(A) n (K)NIEFFEARR(A) (K)SEVRLPNGVLR(T) (K)AGDEGKLPGSIG71UD) Ipdl 66 115158 å¾ä»2-DEè¶ä¸åä¸æç¹çè¶ä¸é ¶åè½è´¨è°±å¾ æç¹ç¨ProGeståproMSMALDIæºå¨äººï¼GSI)èåå¤çãèç½è´¨å¤§çº¦Ipmd,âè马æ¯èæè²ã质谱å¾ç± Voyager DE-RP Pro MALDI-TOF质谱仪ï¼PE BioSystems)åæï¼è§Parker et al., 1998)ãç¨Protein prospectorç¨åºå¨ä¸ç»´ç½ä¸æ£ç´¢SWISS PROTæ°æ®åºï¼hup: //prospector, ucsf. edu),æå¾æ¾ç¤ºçæ¯è´¨éç²¾ 确度åæ¯ä¸ä¸ªå¹é è½æ®µçåºåï¼ä¹æ¾ç¤ºäºèç½é´å®çå¯è½ç»æãèç½åè¶å离ååææ¡ä»¶è§Lopez and Patton. 1999,ä¸åä¹å¤å¨äºä½¿ç¨äº Biolmage 2-Dåæ软件çï¼GSI)ã 7.质谱çæ¥å£ç-ç¥ - ç®åæ两ç§å°è´¨è°±æ´åå ¥èç½è´¨ç»ç 究ç¨åºçæ¹æ³ã为äºé´å®é£äºå¨åç§åºå ç»å èç½è´¨ç»æ°æ®åºä¸-ä¸å-å¨çèç½è´¨ï¼å¿ é¡»è¦ç¡®å®å ¶æ°¨åºé ¸åºåã以å¾æ¯ç¨èç½è´¨åºå仪 éè¿Edmanåå-¦é解æ¹æ³æ¥å®ç°çãè¿ä¸æ-¥éª¤èæ¶é¿ï¼ä¸éè¦ç¸å½å¤§éçèç½è´¨ (Tempst etal., 1999)ãæè¿å¤æ°ç 究è å·²ç»éç¨åæææçµå·é¾ä
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