. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 208 MILTON FINGERMAN AND K. RANGA RAO a black background. Ethanol-soluble fractions of 50 eyestalks of Uca and SO eye- stalks of Palaemonetes were then prepared. During the extraction vigorous stir- ring was employed so as to liberate the white pigment-dispersing substance from the heavy component. After filtering these extracts through Bio-Gel P-6 fraction 12 was boiled as in the previous experiment and assayed for white pigment-dis- persing activity. Each experiment was repeated once and the averaged results are shown in F
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 208 MILTON FINGERMAN AND K. RANGA RAO a black background. Ethanol-soluble fractions of 50 eyestalks of Uca and SO eye- stalks of Palaemonetes were then prepared. During the extraction vigorous stir- ring was employed so as to liberate the white pigment-dispersing substance from the heavy component. After filtering these extracts through Bio-Gel P-6 fraction 12 was boiled as in the previous experiment and assayed for white pigment-dis- persing activity. Each experiment was repeated once and the averaged results are shown in Figure 6. The white pigment-dispersing substance in the ethanol- soluble fraction of the eyestalks from Uca as well as Palaemonetes is thermo- labile, whereas the white pigment-dispersing substance in an extract of these eye- 25 20 t/) 10 0. B FIGURE 6. The white pigment-dispersing Standard Integrated Repsonses (SIR) evoked by the unboiled (solid bars) and boiled (empty bars) fractions retarded by Bio-Gel P-6. Distilled water eluates of the ethanol-soluble fraction of the eyestalks from Palaemonetes (A) and Uca (B) ; distilled water extracts of the eyestalks from Palaemonetes (C) and Uca (D). stalks prepared directly in water is thermostable. The most logical explanation of these results is that we are dealing with at least two different white pigment- dispersing subsances; one, thermostable, is extractable in distilled water, the other, thermolabile, is extractable in ethanol. Previous investigation (Rao, Fingerman and Bartell, 1967) revealed that the white pigment-dispersing substance antagonizes the action of the white pigment- concentrating substance. If a white pigment-concentrating substance is present in the ethanol-soluble fraction of eyestalks from Uca in spite of the fact that its presence is not revealed following injection of such extracts into eyestalkless crabs (Fig. 1), and if it is thermostable, its presence should become apparent by selec-. Please note that thes
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