. Carnegie Institution of Washington publication. 52 FEEDING EXPERIMENTS WITH ISOLATED FOOD -oio =J dead 240 Chart XXI.—Rat 73 fed 181 days on Casein and Zein, and for 53 days on Pea-Legumin as the only proteins. See p. 50. Numbers on Body-weight line indicate time at which each period began. decline sufficient to explain their ultimate death. This fact strongly suggests that death was not necessarily attributable to any primary nutritive defect, but rather to incidental causes. Occasional fatali- ties will not surprise those who have experienced the difficulty of protecting a lar
. Carnegie Institution of Washington publication. 52 FEEDING EXPERIMENTS WITH ISOLATED FOOD -oio =J dead 240 Chart XXI.—Rat 73 fed 181 days on Casein and Zein, and for 53 days on Pea-Legumin as the only proteins. See p. 50. Numbers on Body-weight line indicate time at which each period began. decline sufficient to explain their ultimate death. This fact strongly suggests that death was not necessarily attributable to any primary nutritive defect, but rather to incidental causes. Occasional fatali- ties will not surprise those who have experienced the difficulty of protecting a large number of rats, under the conditions noted, from the appearance of infectious or parasitic maladies which may become fatal. In the same surroundings sudden death has also come to not a few of our animals on ordinary mixed diets consisting of seeds and vegetables. In some cases obvious causes were revealed at autopsy; but systematic post-mortem examinations have not been attempted. With young rats fed similarly we have succeeded in maintaining weight, although with little if any growth. In our preliminary studies of growing animals the food intake was not determined with appropriate care to correlate our findings with the altered curves of growth; hence the insufficient diet rather than any chemical deficiency may have been a possible cause of arrested development. Rats of 30 grams initial weight have been kept by us for many days without gaining weight when fed with a mixture containing a single protein; with desiccated milk in the food they subsequently attained a perfectly normal growth. Despite the obstacles encountered we are inspired to the belief that with modifications in the feeding suggested by our first year's experiments still further progress can be made. Meanwhile further conclusions respecting the inadequacy of the individual proteins for nutritive functions are not Please note that these images are extracted from scanned page images that may have
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