. Manual of bacteriology. Bacteriology. 68 METHODS OF CULTIVATION OF BACTERIA. consequent upon inoculating and sealing procedures; if after incubation no growth occurs in the broth outside the capsule, it is then ready for insertion into the abdominal cavity of the animal. Hanging-drop Cultures. — It is often necessary to observe micro-organisms alive, either to watch the method and rate of their multiplication, or to investigate whether or not they are motile. This is effected by making hanging-drop cultures. The method in the form to be described is only suitable for aerobes. For this specia
. Manual of bacteriology. Bacteriology. 68 METHODS OF CULTIVATION OF BACTERIA. consequent upon inoculating and sealing procedures; if after incubation no growth occurs in the broth outside the capsule, it is then ready for insertion into the abdominal cavity of the animal. Hanging-drop Cultures. — It is often necessary to observe micro-organisms alive, either to watch the method and rate of their multiplication, or to investigate whether or not they are motile. This is effected by making hanging-drop cultures. The method in the form to be described is only suitable for aerobes. For this special slides are necessary. Two forms are in use and are shown in Fig. 34. In A there is ground out on one surface A W/.'////////////////////} -//////^///^/^/^/^^^//,>/7^ B vyy/yy/yAy/yy/y;4^yyyyy^Aw,';'ii>i^^. Fig. 34. A, A. Hollow-ground slide for hanging-drop cultures shown in plan and section. B. Another form of slide for similar cultures. a hollow having a diameter of about half an inch. That shown in B explains itself. The slide to be used and a cover-glass are sterilised by hot air in a Petri's dish, or simply by being heated in a Bunsen and laid in a sterile Petri to cool. In the case of A one or other of two manipulation methods may be employed, (i) If the organism be growing in a liquid culture, a loop of the liquid is placed on the middle of the under surface of the sterile cover-glass, which is held in forceps, the points of which have been sterilised in a Bunsen flame. If the organism be growing in a solid medium, a loopful of sterile bouillon is placed on the cover-glass in the same position, and a very small quantity of the culture (picked up with a platinum needle) is rubbed up in the bouillon. The edge of the hollow is smeared with vaseline, or immersion oil, the cover is then carefully lowered over the. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of t
Size: 2732px × 915px
Photo credit: © The Book Worm / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1900, booksubjectbacteri, bookyear1903
