. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. Acrididae 293 lay eggs during a period of several weeks, in March, April and May, depending on the latitude and season. The nymphs which have hiber- nated become adults, mate, and lay eggs some weeks later. The southern species give about four generations a year in the green- house. They probably do not have a definite hibernating period in their natural habitats. Paratettix texanus, Apotettix eurycephalus, Tettigidea lateralis, Telmatettix aztecus, and Acrydi
. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. Acrididae 293 lay eggs during a period of several weeks, in March, April and May, depending on the latitude and season. The nymphs which have hiber- nated become adults, mate, and lay eggs some weeks later. The southern species give about four generations a year in the green- house. They probably do not have a definite hibernating period in their natural habitats. Paratettix texanus, Apotettix eurycephalus, Tettigidea lateralis, Telmatettix aztecus, and Acrydium arenosum have been bred in the greenhouse at the Kansas Ag- ricultural Experiment Station. The first two mentioned have been bred extensively. A. eurycephalus, due to factors which have not been ascertained, breeds better than any of the others used. All, except A. arenosum and some of the T. lateralis, were from stocks secured in southern Louisiana and Texas, and the region of Tampico, Mexico. They are bred best in a green- house laboratory, with the tem- perature ranging around 8o° F. A variety of cages may be used, but 8" x 12" glass cylinders, with lids of 16-20 mesh wire, set in steam sterilized loam in bulb pots, serve very well. Sterilized sand is placed in the lower part of the pot, and around the cylinder. A smaller empty pot is placed upside down over the hole in the bottom of the bulb pot. It is supposed to aid in aerating the soil, and the food is. Fig. 64.—Cage for rearing grouse locusts and apparatus for transferring progeny. (Transfer apparatus developed by Edgar Millenbruch.) The glass cylinder is 8" in diameter and 12" high, a, suction con- nected with water air pump or sweeper; b, cheesecloth; c, glass chamber into which progeny are sucked; d, the glass cage (covered with 16- to 20-mesh wire screen lid); e, tape; f, corks; g, sand; h, bulb pot; i, loam; k, inverted pot; m. air chamber; n, algae. placed on its extension above the soil
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