. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . Fig. 55.—-Well-distributed coloniesIn agar in portioD of Petri dish. Fig. -Wolffhugels apparatus for countingcolonies. give the total number. This is multiplied by the dilution used in this dish,which gives the colonies developing from 1 of the material used for dishes of another size are used, the area in square centimeters is determinedby multipljring the diameter by lis THE CiJLflVAfldN OF MICROORGANISMS Methods of Inoculating Culture Media.—The inoculation of platin
. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . Fig. 55.—-Well-distributed coloniesIn agar in portioD of Petri dish. Fig. -Wolffhugels apparatus for countingcolonies. give the total number. This is multiplied by the dilution used in this dish,which gives the colonies developing from 1 of the material used for dishes of another size are used, the area in square centimeters is determinedby multipljring the diameter by lis THE CiJLflVAfldN OF MICROORGANISMS Methods of Inoculating Culture Media.—The inoculation of plating madidhas been described under the methods of isolating pure cultures. Platinumwire is usually employed for transferring the growth from one medium toanother. The wire is either attached to the end of a glass rod or to specialaluminum holders which are made for this purpose. Straight wire or wirewith the end bent into a loop is used. The wire is sterilized by heating it inthe flame of a Bunsen burner until red-hot. Care must be taken that the wireis cool before it is used. The straight
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