. A text-book of mycology and plant pathology . Plant diseases; Fungi in agriculture; Plant diseases; Fungi. LABORATORY AND TEACHING METHODS 64s quantities of a pure culture to a stem of young sweet corn and then pricking it in by means of a sterile needle. Some have inoculated the young sweet corn plants by placing the organism in the drops of water which exude from the tips of the corn leaves early in the morning, but the inoculation by means of needle pricks is more certain. Sections should be made of the stem at various stages of growth after inoculation. This is done by using a number of
. A text-book of mycology and plant pathology . Plant diseases; Fungi in agriculture; Plant diseases; Fungi. LABORATORY AND TEACHING METHODS 64s quantities of a pure culture to a stem of young sweet corn and then pricking it in by means of a sterile needle. Some have inoculated the young sweet corn plants by placing the organism in the drops of water which exude from the tips of the corn leaves early in the morning, but the inoculation by means of needle pricks is more certain. Sections should be made of the stem at various stages of growth after inoculation. This is done by using a number of plants. Free-hand sections, or paraffin sections, will show the presence of the organism in the vascular bundles. Stain with carbol fuchsin (Duggar, pp. 111-113)-. Fig. 228.—Arrangement of experiment for inoculation of pear twigs with blight organism, Bacillus amylovorus. LESSON 31 Black-rol of Cruciferous Plants {Bacterium {Pseudomonas) campesiris, Pammel) (see Smith, Erw. Bacteria in Relation to Plant Diseases, pp. 300-334; Duggar, B. M.: pp. 107-111).—This organism is best inoculated into the stem of young cabbage plants below the upper last three leaves, because of the tendency of these leaves to drop oft before the disease has progressed to its fullest extent. The stem is first washed, the organism is smeared on at the point of inoculation and pricked by a sterile cambric needle into place. It is recommended that several sections l)e made, and that to secure the several stages, a number of different inoculations be Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Harshberger, John W. (John William), 1869-1929. Philadelphia : P. Blakiston's Son & Co
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectfungi, bookyear1917
