. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. K. S. YOON ET AL. Figure 3. (A) Immunofluorescence labeling of gut epithelium (e) in cross sections of the tail of Notomuslus lohatus. A mixture ot polyclonal antibodies to the heme protein and davoprotein subunits of chloroper- oxidase was used; binding was visualized by FITC-conjugated anti-rabbit antibody. (B) Neighboring section stained with hemotoxylin and eosin and examined with light microscopy (e—gut epithelium). Scale bar = 72 jtm. highest enzyme activity was detected in extracts from the tail region, with much less
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. K. S. YOON ET AL. Figure 3. (A) Immunofluorescence labeling of gut epithelium (e) in cross sections of the tail of Notomuslus lohatus. A mixture ot polyclonal antibodies to the heme protein and davoprotein subunits of chloroper- oxidase was used; binding was visualized by FITC-conjugated anti-rabbit antibody. (B) Neighboring section stained with hemotoxylin and eosin and examined with light microscopy (e—gut epithelium). Scale bar = 72 jtm. highest enzyme activity was detected in extracts from the tail region, with much less in extracts from the mid-body and head regions (Table I). In addition, the amount of brominated aromatic compounds found in methanol ex- tracts of each body region was measured by GC (Table I). The highest concentration of bromophenols was found in the tail, with a lower level in the mid-body (Table I) and only trace amounts in the head region. Discussion Chloroperoxidase and its products are localized pri- marily in the tail region of Notomastus lobatus (Table I). Immunohistochemistry revealed that the chloroperoxi- dase was located in muscle, epidermis, and gut lining of the tail region as well as to a lesser extent in the mid-body (Figs. 2 and 3). This corresponds closely with the data on the distribution of the natural broi linated products of this chloroperoxidase (4-bromophenol, 2,4-dibromo- phenol, and 2,4,6-tribromophenol), which are also con- centrated in the tail region (Table I). The tail region has times more bromophenol than the mid-body and times more than the head region (bromophenol expressed as micrograms per milligram of protein). In fact, so little bromophenol was detected in the head region that it may represent contamination of the external body surface with bromophenols released from the tail or the mid-body when the worm was collected. Regional distribution of enzyme activity was consistent with these results. The tail showed a very high level
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