Quarterly journal of microscopical science . • , OSTRTH PZ 3- N^^V-^^^^r--^* -F0L OC CF 14 OK. Note on the Comparative Effects on Tissues ofIsotonic Saline and Distilled Water whenused as Solvents for Mercuric Chloride andFormol in Histological Fixation. ByH. M. Carleton, Lecturer in Histology, University of Oxford.(From the Department of Physiology.) Introductory. Should a simple fixative such as mercuric chloride or formolbe dissolved in normal (i. e. isotonic) saline or in water ?Curiously enough, observations are lacking on this simple butfundamental point. Gustav Mann (6) states that form
Quarterly journal of microscopical science . • , OSTRTH PZ 3- N^^V-^^^^r--^* -F0L OC CF 14 OK. Note on the Comparative Effects on Tissues ofIsotonic Saline and Distilled Water whenused as Solvents for Mercuric Chloride andFormol in Histological Fixation. ByH. M. Carleton, Lecturer in Histology, University of Oxford.(From the Department of Physiology.) Introductory. Should a simple fixative such as mercuric chloride or formolbe dissolved in normal (i. e. isotonic) saline or in water ?Curiously enough, observations are lacking on this simple butfundamental point. Gustav Mann (6) states that formol should be diluted withisotonic saline because watery solutions cause such tissues asblood corpuscles and the central nervous system to swell upin whatever strength formol may be used . That, moreover,is the only reference based (apparently) on actual observationwhich has come to my notice. Most authorities on microscopical technique (Langeron, 3;Mallory and Wright, 5) advise that solutions of mercuricchloride and formol be prepared in distilled water, while Lee (4)advocates the dilution
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