. Bulletin. Natural history; Natural history. 290 Illinois Natural History Survky Bulletin Vol. 26. Art. 3 tures for several days in transferring them to the lahoratory. the question arose con- cerning the possible efifects of such tem- peratures on spore germination. Mycelial mats of Eridoc'tiiidiophora fagacearum were grown in the laboratory in the fol- lowing manner. Oak wood pieces meas- uring about 3 inches by inch were placed on end in 250-ml. Erlenmeyer flasks which contained a wheat bran broth (8 gm. of wheat bran in 75 ml. of water). The bran broth was steam sterilized, and, when


. Bulletin. Natural history; Natural history. 290 Illinois Natural History Survky Bulletin Vol. 26. Art. 3 tures for several days in transferring them to the lahoratory. the question arose con- cerning the possible efifects of such tem- peratures on spore germination. Mycelial mats of Eridoc'tiiidiophora fagacearum were grown in the laboratory in the fol- lowing manner. Oak wood pieces meas- uring about 3 inches by inch were placed on end in 250-ml. Erlenmeyer flasks which contained a wheat bran broth (8 gm. of wheat bran in 75 ml. of water). The bran broth was steam sterilized, and, when it was cool, a few drops of a spore suspension of the fungus were placed in. Fig. 8.—Germination of pad cells and conidia of Endoconidiopliora fagacearum. A. Group of gernriinating pad cells producing abundant endoconidia, X125. E. Small germinating pad cell producmg endoconidia after 12 hours at 22 degrees C, X650. C. Four germ tubes extending from a single pad cell, X800. D. Germinating pad cell with abundant sporulation after 48 hours at 28 degrees C, X650. E. Secondary sporulation by germinating conidia after 48 hours at 25 degrees C, Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Illinois. Natural History Survey Division. Urbana, State of Illinois, Dept. of Registration and Education, Natural History Survey Division


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