. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. RECOVERY OF HEART-BEAT OF FUNDULUS EMBRYOS. l6l In an attempt to confirm Loeb's statement (i) that " KG can- not diffuse out of Fnndnlus' egg in distilled water " and assum- ing that it does diffuse in instead of remaining at the vitelline membrane, we endeavored to discover why the heart-beat recov- 10 20 30 40 50 60 70 80 90 100 6_. RECOVERY-DAYS. <7o MORTALITY % VARIATION PERIOD OF RECOVERY 1 3 5 7 9 11 13 15 17 19 FIG. 4. Shows relationship between time for recovery in distilled water and length of exposure
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. RECOVERY OF HEART-BEAT OF FUNDULUS EMBRYOS. l6l In an attempt to confirm Loeb's statement (i) that " KG can- not diffuse out of Fnndnlus' egg in distilled water " and assum- ing that it does diffuse in instead of remaining at the vitelline membrane, we endeavored to discover why the heart-beat recov- 10 20 30 40 50 60 70 80 90 100 6_. RECOVERY-DAYS. <7o MORTALITY % VARIATION PERIOD OF RECOVERY 1 3 5 7 9 11 13 15 17 19 FIG. 4. Shows relationship between time for recovery in distilled water and length of exposure to M/2 KC1 for hearts of Fundulus embryos. The percentage mortality and percentage variation for same eggs are also indi- cated. ered in distilled water. Three lots of 200 eggs each were se- lected, washed and placed in M/2 KG for six, eight and twelve hours, respectively. Then each lot of eggs was washed and put into 40 mis. of distilled water (in a 200 X 25 mm. test tube) changed daily. The daily increase in electrical conductivity was followed. (Before each conductivity measurement the water was boiled momentarily to free it from CCX). Limited time made necessary the omission of a quantitative chemical analysis of the K content but conductivity measurements furnish a possible indi- cation of the K extruded. The graph of Fig. 5 shows that some electrolytic substance was extruded through or at least given off from the membranes of the egg. The fact that the hearts began to beat again leads one to sup- pose that it is at least partly KG. With one exception there was found to be a daily increase in the rate of extrusion. The rate increased most just before recovery of the hearts began to be manifest. The amount extruded was dependent on, but not pro- portional to the length of immersion in M/2 Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not p
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
