. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 448 C. M. Nil AND L. MUSCATINE pellet was resuspended in 3 ml of FSW preheated to 32°C. This suspension was then returned to the incuba- tion chamber for oxygen flux measurements in the light (300 ME/m2/s) for another 60 min at 32°C. Algal dark respiration was measured at the beginning and end of the 60-min period. Oxygen flux for freshly isolated algae is expressed as n\ O2/h/106 cells. The concentration of F1Z was determined using a hemacytometer (Spencer Bright- line) and the mean of eight separate cell counts. Statistica
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 448 C. M. Nil AND L. MUSCATINE pellet was resuspended in 3 ml of FSW preheated to 32°C. This suspension was then returned to the incuba- tion chamber for oxygen flux measurements in the light (300 ME/m2/s) for another 60 min at 32°C. Algal dark respiration was measured at the beginning and end of the 60-min period. Oxygen flux for freshly isolated algae is expressed as n\ O2/h/106 cells. The concentration of F1Z was determined using a hemacytometer (Spencer Bright- line) and the mean of eight separate cell counts. Statistical analyses Nonparametric statistical methods were used for all analyses because some data subsets within the same ex- periment were heteroscedastic (Fma, test, (Sokal and Rohlf. 1981) and Kolmogorov-Smirnoff analysis (Zar, 1984)). Homoscedastic data sets were also analyzed us- ing parametric methods; in these cases, parametric and nonparametric analyses led to identical conclusions. Formazan production by anemones was evaluated for the effects of temperature, light, and "condition" (sym- biotic versus aposymbiotic) by a nonparametric three- way analysis of variance (Zar, 1984). The effect of DCMU on NBT reduction in intact symbiotic and apo- symbiotic anemones was evaluated separately because DCMU effects were investigated only in the light and not in darkness. Including DCMU results would cause an unbalanced analysis of the results obtained for the other treatments (light/temperature/condition). Treatment effects were evaluated at a significance level of Where significant treatment effects occurred, a non- parametric multiple comparison test based on a modifi- cation of the Kruskal-Wallis method (Zar, 1984) was ap- plied at the significance level to identify individual differences among data sets. The Mann-Whitney (/-test was used to determine differences in antioxidant enzyme activities of symbiotic and aposymbiotic animal tissue homogenates and in NBT
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