. A text-book of bacteriology. Bacteriology. EXPERIMENTS UPON ANIMALS. 97 Inoculations are made directly into the circulation through a vein, into the subcutaneous connective tissue, or into one of the serous cavities—usually the peritoneal. The ordinary hypodermic syringe may be used in making injec- tions, but this is difficult to sterilize on account of the leather piston, and complications are liable to arise from its use which it is best to avoid. The best way to sterilize a piston syringe is to wa'sh it thor- oughly with a solution of bichloride of mercury of 1 :1,000, and then to remove
. A text-book of bacteriology. Bacteriology. EXPERIMENTS UPON ANIMALS. 97 Inoculations are made directly into the circulation through a vein, into the subcutaneous connective tissue, or into one of the serous cavities—usually the peritoneal. The ordinary hypodermic syringe may be used in making injec- tions, but this is difficult to sterilize on account of the leather piston, and complications are liable to arise from its use which it is best to avoid. The best way to sterilize a piston syringe is to wa'sh it thor- oughly with a solution of bichloride of mercury of 1 :1,000, and then to remove every trace of bichloride by washing in alcohol. But one never feels quite sure that the most careful washing will insure steril- ization, and it is best to use a syringe which may be sterilized by Fie. 71. heat, such as that of Koch, shown in Fig. 71. In this the metal point and glass tube are easily sterilized in a hot-air oven. Fluid is drawn into the syringe and forced out of it by a rubber ball which has a perforation to be covered by the finder. The writer has for some years boen in the habit of making injec- tions in animals with an improvised glass syringe. This is made from a piece of glass tubing in the same form as the collecting tubes heretofore described. A bulb is blown at one end of the tube, and the other end is drawn out to form a slender tube which serves as the. Fig. 73. needle of the syringe (Fig. 7;^). Bj^ gently heating the bulb in an alcohol lamp and immersing the open end of the capillary tube in the fluid to be injected, this rises into the syringe as the expanded air cools. Having introduced the glass point beneath the skin or into the cavity of the abdomen of the animal to be injected, the contents of the tube are forced out by again heating the bulb by means of a small alcohol lamp. The glass point is easily forced through the thin skin of a mouse or of a young rabbit; but for animals with a thicker skin it is necessary to cut through, or nearly t
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Keywords: ., bookcentury1900, bookdecade1900, booksubjectbacteri, bookyear1901
