. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Grow out Tank. Figure 1. Flow-though culture tanks and grow-out facility. Tank sizes should allow for free, unrestricted feeding and movements of specimens. Mesh sizes should he smaller than the smallest dimension of the organism. brush. Razor blades or narrow-tipped utility knives are helpful for scraping polyps off smooth, flat surfaces such as glass slides. Once removed, polyps are placed into separate tanks and allowed to resettle. Many species reattach quickly when simply resting on the bottom of a dish; others may take
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Grow out Tank. Figure 1. Flow-though culture tanks and grow-out facility. Tank sizes should allow for free, unrestricted feeding and movements of specimens. Mesh sizes should he smaller than the smallest dimension of the organism. brush. Razor blades or narrow-tipped utility knives are helpful for scraping polyps off smooth, flat surfaces such as glass slides. Once removed, polyps are placed into separate tanks and allowed to resettle. Many species reattach quickly when simply resting on the bottom of a dish; others may take longer. One method for raising these polyps off the bottom to facilitate feeding is to tie a tight loop of small- gauge monofilament line around, or slip a small rubber band over, a glass microscope slide, and then insert the base of the polyp under the line on the flat portion of the microscope slide (Groat et al., 1980; F. Boero, Universita di Lecce, Italy, pers. comm.). The tension of the monofilament line holds the polyp next to the surface of the slide without cutting through the stalk of the polyp. The microscope slides can then be inverted, allowing the polyp's tentacles to hang freely. After several days to weeks, the polyp will attach to the slide, and the monofilament can be cut and removed. Asexual reproductive bodies, such as cysts and frustules, can also be removed from the original tank to seed a replicate culture. Cysts can be removed by scraping, and the damage caused to the capsule of the cyst sometimes stim- ulates excystment and subsequent growth of the polyp, as can changes in temperature (Brewer and Feingold, 1991). Swimming frustules are produced in some species (hydroid example: Craspedacusta; scyphozoan example: certain rhi- zostomes such as Cassiopeia and ), and these can be pipetted into a dish where they will settle and develop into polyps. After settlement, the dish can be transferred to a flow-through tank. The use of antibiotics to aid
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
