. Cytochemistry: a critical approach. Cells. ALDEHYDES AS CHROMOGENIC REAGENTS 105 4. Sections are washed in two changes of N hydrochloric acid for 3 or 4 minutes, and transferred to 5. A nitrous acid solution obtained by dissolving gram of sodium nitrite in 100 milliliters N hydrochloric acid kept at 0° C. The sec- tions are kept in this solution for 5 minutes to diazotise the amino groups. 6. The sections are transferred to an alkaline solution of a phenol or a solution of an aromatic amine, , H acid or a-naphthol or /3-naphthyla- mine. After 15 minutes in this solution coupl
. Cytochemistry: a critical approach. Cells. ALDEHYDES AS CHROMOGENIC REAGENTS 105 4. Sections are washed in two changes of N hydrochloric acid for 3 or 4 minutes, and transferred to 5. A nitrous acid solution obtained by dissolving gram of sodium nitrite in 100 milliliters N hydrochloric acid kept at 0° C. The sec- tions are kept in this solution for 5 minutes to diazotise the amino groups. 6. The sections are transferred to an alkaline solution of a phenol or a solution of an aromatic amine, , H acid or a-naphthol or /3-naphthyla- mine. After 15 minutes in this solution coupling with the diazotised sec- tions should be complete. 7. The sections are washed in tap water and mounted in balsam. Table VIII gives a list of some of the nitro reagents which are usually available and indicates the groups of proteins for the study of which they are particularly suitable. Aldehydes as Chromogenc Reagents A third family of compounds useful in the cytochemistry of proteins are those containing two reactive groups, one of which is an aldehyde group and the other of which may be any of a TABLE IX Aldehyde reagents under investigation for the cytochemistry of and —NH2 groups. -SH. CHO CHO CHO o NH, CHON02 CHO^ >COCH2Br CHO >0-CO-CH2I + + + CHO o H • CO • CH2I SH + + +. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Danielli, J. F. (James Frederic), 1911-. New York Wiley
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