. A manual of bacteriology for agricultural and general science students. Bacteriology. 30 A MANUAL OF BACTERIOLOGY Exercise 54. Preparation of Czapek's Nutrient Solution for the Cultivation of Fungi Distilled water oc. Magnesium sulphate g. Dipotassium phosphate . g. Potassium chloride g. Ferrous sulphate ... . . g. Sodium nitrate . . . g. Cane sugar g. Exercise 55. Preparation of Sulphindigotate Bouillon (Weyl) for Detecting Anaerobic Organisms To 1 liter of regular beef bouillon add 20 g. of glucose and 1 g. of sodium sulphindigotate. After sterilizati
. A manual of bacteriology for agricultural and general science students. Bacteriology. 30 A MANUAL OF BACTERIOLOGY Exercise 54. Preparation of Czapek's Nutrient Solution for the Cultivation of Fungi Distilled water oc. Magnesium sulphate g. Dipotassium phosphate . g. Potassium chloride g. Ferrous sulphate ... . . g. Sodium nitrate . . . g. Cane sugar g. Exercise 55. Preparation of Sulphindigotate Bouillon (Weyl) for Detecting Anaerobic Organisms To 1 liter of regular beef bouillon add 20 g. of glucose and 1 g. of sodium sulphindigotate. After sterilization, which should be done in the Arnold sterilizer, the medium should be blue. The growth of anaerobic organisms turns it yellow. Solid media may be had by the addi- tion of gelatin or agar. Exercise 56. Methods of Cultivating Anaerobic Bacteria These bacteria grow in an atmos- phere devoid of oxygen, obtaining the oxygen necessary for their meta- bolism from carbohydrates and other compounds in the medium which they break down. Any method of culture is successful which either absorbs the gaseous oxygen present or replaces the air with a nonpoisonous gas. 1. Test-tube cultures. The cultures should be made in dextrose beef agar or gelatin, which should be freshly prepared and always boiled to expel air immediately before being inocu- lated. Wright's modification of Buchner's method, given on the following page, is very Fig. 16. Novy jar for anaerohic cultures The jar is sealed by turning the stopper in the top. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Reed, Howard Sprague, 1876-. Boston, New York, Ginn
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