. Pathogenic micro-organisms. A text-book of microbiology for physicians and students of medicine. (Based upon Williams' Bacteriology). Bacteriology; Pathogenic bacteria. 378 SPECIFIC MICRO-ORGANISMS as are all the spirochetes, and has a very slender flagellum at each end. The usual motion is that of rapid rotation on the longitudinal axis with progression, but at times there is gross bending of the filament, especially when the organism is living under unfavorable conditions. The mode of division is a somewhat vexed question as it is in regard to the whole group of spirochetes. Transverse and
. Pathogenic micro-organisms. A text-book of microbiology for physicians and students of medicine. (Based upon Williams' Bacteriology). Bacteriology; Pathogenic bacteria. 378 SPECIFIC MICRO-ORGANISMS as are all the spirochetes, and has a very slender flagellum at each end. The usual motion is that of rapid rotation on the longitudinal axis with progression, but at times there is gross bending of the filament, especially when the organism is living under unfavorable conditions. The mode of division is a somewhat vexed question as it is in regard to the whole group of spirochetes. Transverse and longi- tudinal division have been de- scribed. Probably the weight of authority^ now favors transverse division as the sole mode of multi- plication, although able adherents to the opposite view are not lack- ing. The refractive index of the filament is not very much greater than that of serum, so that the unstained organism is difficult tq see by direct illumination. Dark- field illumination is more satis- factory. Sp. pallida in film prep- arations stains with difficulty by ordinary methods. Schaudinn em- ployed Giemsa's modification of the Romanowsky stain. Good re- sults are obtained by staining with solutions of the Romanowsky staining principles in methyl alcohol provided an excess of me- thylene-violet be present (see p. 44). Tunninclifl^ recom- mends staining with a mixture of saturated alcohoHc solution of gentian violet, i part, in 5 per cent carbolic acid, 9 parts. Thin films are essential but staining process requires only a few Fig, 151.—rFilm preparation from a genital syphilitic papule; in the center are two specimens of Spiro- chceta pallida, the other three are specimens of Spirochata refringens. (After Schaudinn and Hoffmann.) ' Journ. Exp. Med., 1911, Vol. XIV, p. 99; 1912, Vol. XV, p. 90. ^Journ. A. M. A., 1912, Vol. LVIII, p. Please note that these images are extracted from scanned page images that may have been digitally enhanced for re
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