Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . bercle bacilli in sputum; carbolfuchsin and methylene-blue(Zeiss one-twelfth oil-immersion). Origin.—In various tuberculous products of man and otheranimals and in the dust containing the discharges. Form.—^Very slender rods, sUghtly curved, 2 /x to 4 ju inlength, about one-quarter the size of a red blood-corpusclesdiameter, their ends rounded, usually solitary, often, how-ever, lying in pairs in such a manner as to form an acuteangle. Sometimes they are S-shaped. In co
Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . bercle bacilli in sputum; carbolfuchsin and methylene-blue(Zeiss one-twelfth oil-immersion). Origin.—In various tuberculous products of man and otheranimals and in the dust containing the discharges. Form.—^Very slender rods, sUghtly curved, 2 /x to 4 ju inlength, about one-quarter the size of a red blood-corpusclesdiameter, their ends rounded, usually solitary, often, how-ever, lying in pairs in such a manner as to form an acuteangle. Sometimes they are S-shaped. In colored prepara- BACILLUS TUBERCULOSIS AND ALLIED ORGANISMS III tions little oval spaces are seen in the rod which resemblespores, but have none of the properties of spores. (See , 48.) Properties.—Does not possess motility. Growth.—Requires special media for its growth, and a tem-perature varying but slightly from ° C. It grows slowly,developing first after ten days, reaching its maximum inthree weeks. It is facultative anaerobic. On gelatin it doesnot form a growth. The media should be slightly acid;. Fig. 48.—Giant-cell containing bacilli (from a photograph made by M. Gray). growth mostly on surface. Subcultures grow more rapidlythan those direct from lesions. Colonies on Blood-serum.—Koch first used blood-serum forculture, and obtained thereon very good growths. Strokecultures or test-tubes inoculated with small bits of tubercu-lar tissue are placed in a well-ventilated and slightly humidincubator at 37° C. for ten to fourteen days, when smaUglistening white points appear, which then coalesce to form adry, white, scale-like growth. Under microscope, composedof many fine lines containing the tubercle bacillus. Glycerin-agar.—By adding 4 to 6 per cent, glycerin to 112 ESSENTIALS OF BACTERIOLOGY ordinary agar-peptone medium, Nocard and Roux obtained aculture-medium upon which tubercle bacilli grow much betterthan upon blood-serum, especially af
Size: 1571px × 1589px
Photo credit: © The Reading Room / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookcentury1900, bookdecade1910, bookpublisherphila, bookyear1913
