. Kirkes' handbook of physiology . of determining the isotonicity of blood. \ COAGULATION OF BLOOD 137 Make up a series of solutions of sodium chloride, varying by tenths, to per cent. Prepare a series of slides with vaseline rings and mountdrops of human blood in drops of saline of , , , , 1, and percent, examine every ten minutes under a high-power microscope. Thecorpuscles of some of the slides will swell up and may disintegrate, otherswill show crenation as in figure 126. In the isotonic solutions the corpus-cles will appear of their normal size and condition. 1
. Kirkes' handbook of physiology . of determining the isotonicity of blood. \ COAGULATION OF BLOOD 137 Make up a series of solutions of sodium chloride, varying by tenths, to per cent. Prepare a series of slides with vaseline rings and mountdrops of human blood in drops of saline of , , , , 1, and percent, examine every ten minutes under a high-power microscope. Thecorpuscles of some of the slides will swell up and may disintegrate, otherswill show crenation as in figure 126. In the isotonic solutions the corpus-cles will appear of their normal size and condition. 10. Coagulation of Blood, a. Normal Clot. Anesthetize a dog,insert a cannula into the carotid or femoral artery, and draw samples ofblood into two or three clean, dry test tubes. Draw one sample into a testtube that has had its sides oiled. Note the exact time at which the bloodwas drawn into the test tubes and set the test tubes in a test-tube rack. Ex-amine at intervals of 30 seconds by gently inclining the test tubes. Presently. Fig. 132.—Miscroscopic View of Clot Showing Fibrin Network. it will be noted that the blood becomes more viscous and does not flow freelyup the sides of the test tubes. Later the whole mass will become jelly-likeand will retain the form of the test tube. Note the time of the first slightchange, and also when the clot becomes more perfect. The sample in theoiled test tube will be found to clot more slowly. If the test tubes of clotted blood are left standing for a day, the coagulumwill become similar in size and a transparent yellowish blood will make itsappearance on the surface or between the sides of the clot and the test-tubewall. This fluid is the serum and it is squeezed out by the shrinking of thefibrin which holds the corpuscles in its meshes. b. Microscopic Examination oj the Process 0} Clotting. Take a drop of 138 THE BLOOD fresh blood from the tip of your finger under sterile conditions and mounton a microscopic slide in a few drops of sal
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