. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. BLOOD PLASMA OF ASCIDIANS 659 absorbance at about 500 nm which imparted the pink color to its plasma. The CD spectrum of the plasma of P. stolonifera is recorded in Figure 1 for comparison with chromatographed constituents of the plasma. The plasmas of both species were chromatographed through Sephadex G75 with NaCl as eluent. The elution patterns detected at 275 nm for both species and also at 325 mn for P. stolonifera are given in Figure 2. For both species two major bands, one each, in fractions 1-2 and 8-13 were ob
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. BLOOD PLASMA OF ASCIDIANS 659 absorbance at about 500 nm which imparted the pink color to its plasma. The CD spectrum of the plasma of P. stolonifera is recorded in Figure 1 for comparison with chromatographed constituents of the plasma. The plasmas of both species were chromatographed through Sephadex G75 with NaCl as eluent. The elution patterns detected at 275 nm for both species and also at 325 mn for P. stolonifera are given in Figure 2. For both species two major bands, one each, in fractions 1-2 and 8-13 were observed. The second band from P. stolonifera could contain several components. In addition, a pink compound in fractions 3-5 was observed between the two major bands. The first band eluted (fractions 1-2) was in the void volume of the column and should have contained compounds with molecular weights greater than the exclusion of the G75 column (30,000). It gave a positive test for protein, and negative tests for N- acetylaminosugar and iron for both species. It had a maximum at 275 nm in the absorption spectrum for P. stolonifera and at 282 for A. ccratodes. Fractions 3-5 differed for the two species. Both give positive tests for protein and negative tests for N-acetylaminosugar and iron. However, the absorption spectrum for P. stolonifera (Fig. 3) contained maxima at 497 and 275 nm, whereas the 497 nm band was missing for A. ccratodes. This pink band from /'. stolonifera was eluted with water in fractions 2-4. The CD spectrum of the pink band had a positive Cotton effect at 516 nm, a negative at 387 nm, and a positive at 293 nm. Below 260 nm the CD was negative. Fractions 8-13 for A. ceratodes had absorption maxima at 260 and 314 nm with the ratio of the absorbance at the two wavelengths increasing as the fraction number increases, suggesting that more than one compound was present. For P. stolonifera, on some occasions only a 327-nm maximum was observed in fractions 8-13, and
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
