. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. VOL. 12 (1953) CO COMPOUNDS OF RESPIRATORY ENZYMES 293 First, the photodissociation is accomplished by illuminating the cell suspension with yellow light, for example, the 589 m/i line of the Na arc or the 578 m/x of the Hg arc, other portions of the spectrum of these line sources being readily eliminated by appro- priate filters having the characteristics shown in Fig. 3. The observation of the photo- dissociation of CO compounds is made in the
. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. VOL. 12 (1953) CO COMPOUNDS OF RESPIRATORY ENZYMES 293 First, the photodissociation is accomplished by illuminating the cell suspension with yellow light, for example, the 589 m/i line of the Na arc or the 578 m/x of the Hg arc, other portions of the spectrum of these line sources being readily eliminated by appro- priate filters having the characteristics shown in Fig. 3. The observation of the photo- dissociation of CO compounds is made in the region of the Soret band (410-480 m/x) and the photocell is rendered insensitive to the yellow light not only by the nature of its surface (Cs-Sb) but also by the blue colour filter combination shown by the solid curve of Fig. 3. '°° 1 C - 3480. 700 A(m;j) Fig. 3. Transmission curves for optical filters used to isolate photodissociating light (above 520 m/n) from spectrophotometric wavelengths (410-480 m/i). C represents Corning glass filters, W represents Wratten filter, and B + L represents Bausch and Lomb interference filters (MD-27). MONOCHROMATOR set to /\. Exit sht MONOCHROMATOR set to T^p 7-\ Vibrating mirror *V :_'; / Color filters Cell (Blue' /." xit slit suspension :D:::::::i:p-^-^: (Entronce ^ slit Ptiotocell AC Amplifier i> o Tungsten lomp =â <-Yellow filter â¢Â«-Shutter ^^\*-Sodium ore Kj) ;\=589m^ Half silvered mirror Fig. 4. A schematic diagram of a double-beam spectrophotometer suitable for measuring the kinetics of the photochemical decomposition of the CO compounds of the respiratory enzymes within intact cells. The characteristics of the optical filters are given in Fig. 3. The wavelengths Aa and Ab are set in the region 410-480 mju (MD-28). Secondly, as Fig. 4 shows, the differential double-beam spectrophotometer em- ploys two wavelengths of light, one a reference wavelength, for example 480 m/x where no appreciable optical density c
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