. Canadian journal of agricultural science. Agricultural Institute of Canada; Agriculture. April, 1922. SCIENTIFIC AGRICULTURE. 261 found very ; The stigma should now be carefully ex- amined witli a lens to see if all pollen has been cleared away; then any drops of water adhering to any part of the flow- er sliould be removed by the use of small strips of thin blotting paper. It was also found that the frayed edge of such paper is very useful for brushing away anthers or pollen grains which may not have been removed by the Avater spray. Wlien a care- ful inspection of the stigma
. Canadian journal of agricultural science. Agricultural Institute of Canada; Agriculture. April, 1922. SCIENTIFIC AGRICULTURE. 261 found very ; The stigma should now be carefully ex- amined witli a lens to see if all pollen has been cleared away; then any drops of water adhering to any part of the flow- er sliould be removed by the use of small strips of thin blotting paper. It was also found that the frayed edge of such paper is very useful for brushing away anthers or pollen grains which may not have been removed by the Avater spray. Wlien a care- ful inspection of the stigma shows that it is quite clear and that all anthers and pollen grains have been removed, cross- pollination may be performed. At this time, the stigma is still held in position by the piece of Timothy .stem and is standing quite free from the stand- ard (See fig. IIIc.) so that little difficulty is experienced in pollinating the stigma. The pollen may be collected on the flat- tened end of a quill or a wooden toothpick and transferred directly to the prepared stigma of the female parent. The tootli- pick is preferred to a camel hair brush as it is more convenient to manipulate and can much more be cleaned or sterili- zed for future use. A careful examination of the stigma should noAv be made to make certain that an abundance of healthy pollen is resting on the stigma (care should be taken to distinguish between pollen grains and empty anther sacs). If found satisfactory, the piece of Timothy stem may be re- moved. In doing this, see that it is re- moved at right angles to the sexual column and in such a way as not to displace the stigma or the adhering pollen. The re- moval of this piece of Timothy stem al- lows the process of tripping to continue until it is completed. The pollen-covered stigma then presses forward and comes in close contact with the standard; thus, tiie pollen grains are entrapped and become firmly attached to the face of the stigma : thus completing tl
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