. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. The Specific Precipitins 121 of lO cc. of. mann,* Morgenroth, and others, by whom it has been shown that the reaction is sufficiently accurate to make possible the differentia- tion of human and goat's milk. The most important practical application of the specific character of the precipitins, however, came through Uhlenhuthf and Wassermann,f who made use of it for the differentiation of bloods for forensic purposes. Uhlenhuth gave rabbits intraperitonea
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. The Specific Precipitins 121 of lO cc. of. mann,* Morgenroth, and others, by whom it has been shown that the reaction is sufficiently accurate to make possible the differentia- tion of human and goat's milk. The most important practical application of the specific character of the precipitins, however, came through Uhlenhuthf and Wassermann,f who made use of it for the differentiation of bloods for forensic purposes. Uhlenhuth gave rabbits intraperitoneal Injections defibrinated blood at intervals of from six to eight days and found the blood-serum strongly precipitant after the fifth. He used such serum for testing the reaction with the bloods of oxen, horses, donkeys, pigs, sheep, dogs, cats, deer, hares, guinea-pigs, rats, mice, rabbits, chickens, geese, turkeys, pigeons, and men. The method of making the test is important, as carelessness of detail will interfere with the accuracy of the result. The blood to be tested is diluted about i: loo, or until it has a feeble red color, with tap water, and then freed from cor- puscular stroma by filtration or decantation. Two cubic centimeters of it are placed in a small test-tube, and further diluted with an equal quantity of physiological salt solution (if more water be added a precipitate of globulin might take place and spoil the experiment). To such a prepared blood solution, from six to eight drops of the immune serum are added. If the diluted blood come from the same kind of animal as that whose blood was used to immunize the animal furnishing the test serum, immediate clouding takes place, and a flocculent precipi- tate forms. The precipitate never occurs with any other blood. Wassermann and Schutze§ prepared a test serum by injecting rabbits with human blood, precipitating powers upon twenty-three other kinds of blood and found no precipitate except with the blood of a ba
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1916
