. Pathological technique; a practical manual for workers in pathological histology and bacteriology. lystained areas in their protoplasm (Fig. 74). The largerforms of the bacillus are usually slightly bent or wavy inoutline. Slight irregularities in shape may be morphology varies considerably on different culture-media. In cover-glass preparations from the lesions the bacilliusually appear somewhat longer and thicker than the tuber-cle bacillus, and show numerous sharply defined, unstainedor faintly stained areas in their protoplasm (Fig. 75). Theyhave rounded or conical ends, and
. Pathological technique; a practical manual for workers in pathological histology and bacteriology. lystained areas in their protoplasm (Fig. 74). The largerforms of the bacillus are usually slightly bent or wavy inoutline. Slight irregularities in shape may be morphology varies considerably on different culture-media. In cover-glass preparations from the lesions the bacilliusually appear somewhat longer and thicker than the tuber-cle bacillus, and show numerous sharply defined, unstainedor faintly stained areas in their protoplasm (Fig. 75). Theyhave rounded or conical ends, and are sometimes slightlyirregular in shape. As a rule, they are present in smallnumbers. If Lofflers methylene-blue solution is used forstaining the cover-glass, it should be heated; if carbol- PATHOGENIC BACTERIA AND FUNGI 315 fuchsin is used, it should be followed by a slight decolori-zation with 95 per cent, alcohol to better differentiate thebacilli. Gram-negative; non-motile. Blood-serum.—Rounded, elevated, colorless, viscid-lookingcolonies, growing slowly and becoming well developed after. Fig. 74.—Glanders bacilli from a young culture on potato ; X 2000 (Wright and Brown).
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